The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.
The Arabidopsis thaliana elongator complex subunit 2 epigenetically affects root development.
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拟南芥伸长复合体亚基 2 表观遗传影响根发育。
DOI：
10.1093/jxb/erv230
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发表时间：
2015-08
影响因子：
6.9
通讯作者：
Ding Z
中科院分区：
文献类型：
--
作者：
Jia Y;Tian H;Li H;Yu Q;Wang L;Friml J;Ding Z
Elp2突变体通过乙酰化或甲基化影响关键转录因子和CyCB1的表达，还改变了生长素的极性运输，降低了根中生长素的含量。伸长复合体亚单位2(ELP2)蛋白是进化上保守的组蛋白乙酰转移酶复合体的一个亚基，已被证明参与拟南芥的叶片形成、植物免疫和非生物胁迫反应。在此，我们探讨了它在根发育中的作用。与野生型相比，elp2突变体表现出根干细胞的加速分化和静止中心(QC)的细胞分裂更活跃。AP2转录因子PLT1(PLETHORA1)和PLT2(PLETHORA2)，GRAS转录因子SCR(稻草人)和SHR(短根)以及WUSCHEL相关的HOMEOBOX5转录因子WOX5都在突变体中强烈下调。另一方面，G2/M转换激活剂CyCB1在elp2中大量诱导表达。生长素外流转运体PIN1和PIN2的蛋白水平降低，PIN1的elp2也表现出轻微的极性变化，导致根尖生长素含量下降。这些基因的乙酰化或甲基化水平在突变体和野生型之间都不同，这表明ELP2对根发育的调控涉及一系列转录因子和其他发育调节因子的表观遗传修饰。
The elp2 mutant affected the expression of key transcription factors and CYCB1 through either acetylation or methylation, and also altered auxin polar transport and reduced auxin content in root. The elongator complex subunit 2 (ELP2) protein, one subunit of an evolutionarily conserved histone acetyltransferase complex, has been shown to participate in leaf patterning, plant immune and abiotic stress responses in Arabidopsis thaliana. Here, its role in root development was explored. Compared to the wild type, the elp2 mutant exhibited an accelerated differentiation of its root stem cells and cell division was more active in its quiescent centre (QC). The key transcription factors responsible for maintaining root stem cell and QC identity, such as AP2 transcription factors PLT1 (PLETHORA1) and PLT2 (PLETHORA2), GRAS transcription factors such as SCR (SCARECROW) and SHR (SHORT ROOT) and WUSCHEL-RELATED HOMEOBOX5 transcription factor WOX5, were all strongly down-regulated in the mutant. On the other hand, expression of the G2/M transition activator CYCB1 was substantially induced in elp2. The auxin efflux transporters PIN1 and PIN2 showed decreased protein levels and PIN1 also displayed mild polarity alterations in elp2, which resulted in a reduced auxin content in the root tip. Either the acetylation or methylation level of each of these genes differed between the mutant and the wild type, suggesting that the ELP2 regulation of root development involves the epigenetic modification of a range of transcription factors and other developmental regulators.