Evaluation of autofocus functions in molecular cytogenetic analysis
Evaluation of autofocus functions in molecular cytogenetic analysis
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DOI：
10.1046/j.1365-2818.1997.2630819.x
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发表时间：
1997-12-01
期刊：
影响因子：
--
通讯作者：
Del Pozo, F
中科院分区：
文献类型：
--
作者：
Santos, A;De Solórzano, CO;Del Pozo, F
这项工作描述了对用于分析荧光图像细胞仪研究的几种自动对焦函数的系统评估。聚焦是细胞自动荧光原位杂交分析的第一步。已经使用定性和定量程序评估了13个函数，对于这些程序的最后一个过程，定义并提出了一个函数。这个新的FOM考虑了聚焦功能的五个重要功能。我们的结果表明，基于相关度量的功能对于此类图像具有最佳性能。
This work describes a systematic evaluation of several autofocus functions used for analytical fluorescent image cytometry studies of counterstained nuclei. Focusing is the first step in the automatic fluorescence in situ hybridization analysis of cells. Thirteen functions have been evaluated using qualitative and quantitative procedures, For the last of these procedures a figure-of-merit (FOM) is defined and proposed. This new FOM takes into account five important features of the focusing function. Our results show that functions based on correlation measures have the best performance for this type of image.