Heparinized PLLA/PLCL nanofibrous scaffold for potential engineering of small-diameter blood vessel: Tunable elasticity and anticoagulation property
Heparinized PLLA/PLCL nanofibrous scaffold for potential engineering of small-diameter blood vessel: Tunable elasticity and anticoagulation property
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用于小直径血管潜在工程的肝素化 PLLA/PLCL 纳米纤维支架：可调弹性和抗凝性能
DOI：
10.1002/jbm.a.35315
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发表时间：
2015-05-01
影响因子：
4.9
通讯作者：
Wang, Guoqing
中科院分区：
文献类型：
--
作者：
Wang, Weizhong;Hu, Jinwei;Wang, Guoqing
组织工程血管移植物的成功在很大程度上取决于合成管状支架，其可以模拟天然血管的结构、机械和抗凝性能。本研究采用热致相分离技术制备了不同重量比的聚左旋乳酸（PLLA）和聚左旋丙交酯-己内酯（PLCL）的小直径管状支架。为了提高材料的抗凝性能，通过N-（3-二甲氨基丙基）-N-乙基碳二亚胺盐酸盐和N-羟基琥珀酰亚胺偶联化学将肝素共价连接到管状支架上。所制备的PLLA/PLCL支架保留了纯PLLA支架中观察到的微孔纳米纤维结构，并且可以通过改变两种组分的比例来微调其结构和力学性能。PLCL含量为60%的支架在弹性和结构完整性方面被认为是最有前途的小直径血管工程支架。肝素化支架的亲水性增强，蛋白质吸附能力降低，体外抗凝血能力增强。猪髂动脉内皮细胞接种在肝素化支架上显示出良好的细胞附着、铺展、增殖和表型维持。此外，肝素化支架在新西兰白色兔皮下植入1个月和2个月后显示出新生血管形成。肝素化PLLA/PLCL纳米纤维支架具有良好的血管组织工程应用前景。(c)2014 Wiley Periodicals，Inc. J Biomed Mater Res Part A：103A：1784-1797，2015.
The success of tissue engineered vascular grafts depends greatly on the synthetic tubular scaffold, which can mimic the architecture, mechanical, and anticoagulation properties of native blood vessels. In this study, small-diameter tubular scaffolds were fabricated with different weight ratios of poly(l-lactic acid) (PLLA) and poly(l-lactide-co--caprolactone) (PLCL) by means of thermally induced phase separation technique. To improve the anticoagulation property of materials, heparin was covalently linked to the tubular scaffolds by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide coupling chemistry. The as-prepared PLLA/PLCL scaffolds retained microporous nanofibrous structure as observed in the neat PLLA scaffolds, and their structural and mechanical properties can be fine-tuned by changing the ratio of two components. The scaffold containing 60% PLCL content was found to be the most promising scaffold for engineering small-diameter blood vessel in terms of elastic properties and structural integrity. The heparinized scaffolds showed higher hydrophilicity, lower protein adsorption ability, and better in vitro anticoagulation property than their untreated counterparts. Pig iliac endothelial cells seeded on the heparinized scaffold showed good cellular attachment, spreading, proliferation, and phenotypic maintenance. Furthermore, the heparinized scaffolds exhibited neovascularization after subcutaneous implantation into the New Zealand white rabbits for 1 and 2 months. Taken together, the heparinized PLLA/PLCL nanofibrous scaffolds have the great potential for vascular tissue engineering application. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1784-1797, 2015.