Combination of hydrostatic pressure and lacticin 3147 causes increased killing of Staphylococcus and Listeria
Combination of hydrostatic pressure and lacticin 3147 causes increased killing of Staphylococcus and Listeria
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静水压和乳酸菌素 3147 的组合可增加对葡萄球菌和李斯特菌的杀灭作用
DOI：
10.1046/j.1365-2672.2000.00975.x
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发表时间：
2000
影响因子：
4
通讯作者：
C. Hill
中科院分区：
文献类型：
--
作者：
S. Morgan;R. Ross;T. Beresford;C. Hill
在牛奶和乳清中评估了静水压和乳酸菌素3147处理的使用，以结合两种处理来改善最小加工乳制品的质量。使用两种食源性病原体：金黄色葡萄球菌ATCC 6538和无害李斯特菌DPC 1770对该系统进行了评价。对葡萄球菌的审判。aureus ATCC 6538的培养，使用从培养物上清液制备的浓缩乳酸菌素3147进行。结果表明，当两种处理组合使用时，其效果大于相加效应。例如，250 MPa（2.2个对数减少）和乳酸菌素3147（1个对数减少）的组合导致超过6个对数的杀灭。 当评价食品级粉末形式的乳酸菌素3147（从喷雾干燥的重组脱矿物质乳清粉发酵剂中开发）对L.无毒DPC 1770。此外，观察到用大于400 MPa的压力处理乳酸菌素3147制剂产生细菌素活性的增加（相当于活性加倍）。 这些结果表明，高压和乳酸菌素3147的组合可能适合于在较低的静水压力水平下改善最小加工食品的质量。
The use of hydrostatic pressure and lacticin 3147 treatments were evaluated in milk and whey with a view to combining both treatments for improving the quality of minimally processed dairy foods. The system was evaluated using two foodborne pathogens: Staphylococcus aureus ATCC6538 and Listeria innocua DPC1770. Trials against Staph. aureus ATCC6538 were performed using concentrated lacticin 3147 prepared from culture supernatant. The results demonstrated a more than additive effect when both treatments were used in combination. For example, the combination of 250 MPa (2.2 log reduction) and lacticin 3147 (1 log reduction) resulted in more than 6 logs of kill. Similar results were obtained when a foodgrade powdered form of lacticin 3147 (developed from a spray dried fermentatation of reconstituted demineralized whey powder) was evaluated for the inactivation of L. innocua DPC1770. Furthermore, it was observed that treatment of lacticin 3147 preparations with pressures greater than 400 MPa yielded an increase in bacteriocin activity (equivalent to a doubling of activity). These results indicate that a combination of high pressure and lacticin 3147 may be suitable for improving the quality of minimally processed foods at lower hydrostatic pressure levels.