Mutagenic effects of carbon ion beam irradiations on dry Lotus japonicus seeds
Mutagenic effects of carbon ion beam irradiations on dry Lotus japonicus seeds
复制标题
碳离子束辐照对干莲子的诱变效应
DOI：
10.1016/j.nimb.2016.06.021
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发表时间：
2016-09-15
影响因子：
1.3
通讯作者：
Chen, Yuze
中科院分区：
文献类型：
--
作者：
Luo, Shanwei;Zhou, Libin;Chen, Yuze
碳离子束辐照是一种有效的诱变方法，在作物育种中的应用越来越广泛。采用80 MeV/u的碳离子束，以0、100、200、300、400、500和600戈伊的剂量辐照莲藕干种子，研究了碳离子束对莲藕干种子的诱变效应。对M-1群体的发芽率、存活率和根长进行了研究，并选择400戈伊作为大规模突变体筛选的半致死剂量（LD 50）。在2472株M-2植株中，共发现叶、茎、花、果实等形态变异127个，变异频率约为5.14%。利用简单重复序列（ISSR）分析方法，研究了M-2群体中7个突变体和8个无表型变异植株的DNA多态性。两组间多态性差异不显著，总多态性率为0.567%。(C)2016爱思唯尔B. V.保留所有权利。
Carbon ion beam irradiation is a powerful method for creating mutants and has been used in crop breeding more and more. To investigate the effects of carbon ion beams on Lotus japonicus, dry seeds were irradiated by 80 MeV/u carbon ion beam at dosages of 0, 100, 200, 300, 400, 500 and 600 Gy. The germination rate, survival rate and root length of M-1 populations were explored and the dose of 400 Gy was selected as the median lethal dose (LD50) for a large-scale mutant screening. Among 2472 M-2 plants, 127 morphological mutants including leaf, stem, flower and fruit phenotypic variation were found, and the mutation frequency was approximately 5.14%. Inter simple sequence repeat (ISSR) assays were utilized to investigate the DNA polymorphism between seven mutants and eight plants without phenotypic variation from M-2 populations. No remarkable differences were detected between these two groups, and the total polymorphic rate was 0.567%. (C) 2016 Elsevier B.V. All rights reserved.