Trypanosoma cruzi (kinetoplastida, Trypanosomatidae) genotypes in neotropical bats in Brazil
Trypanosoma cruzi (kinetoplastida, Trypanosomatidae) genotypes in neotropical bats in Brazil
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DOI：
10.1016/j.vetpar.2008.06.004
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发表时间：
2008-10-01
影响因子：
2.6
通讯作者：
Jansen, Ana Maria
中科院分区：
文献类型：
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作者：
Lisboa, Cristiane Varella;Pinho, Ana Paula;Jansen, Ana Maria
很少有研究调查翼翅目昆虫在克氏锥虫森林传播周期中所起的作用，或它们与该寄生虫主要基因型的推定关联。为了扩大对这一问题的认识，本研究收集了巴西不同地区蝙蝠4科93份标本，分别为Molossidae、Noctilionidae、Phyllostomidae和Vespertilionidae。未见明显的寄生虫血症，但有14%（13/93）的检测样本克氏体血液培养阳性。大部分分离的寄生虫来自塞拉多地区同一棵布里蒂空心棕榈树上捕获的hastatus Phyllostomus（80%）。多位点酶电泳（MLEE）分析表明，各分离株间的遗传距离为0.35，除参考菌株外，其余分离株间的遗传距离均为0.40。酶原体与蝙蝠属、种、起源地理区域均无相关性，且酶原体与寄生虫基因型相关性不强。从蝙蝠获得的14株克氏t型虫分离株中有10株符合TCII基因型。观察到翼目动物有TCI、TCII/TCIII混合感染以及兰氏锥虫单一或混合感染。这些结果表明，蝙蝠可能是克氏锥虫主要基因型（TCI、TCII、TCII/Z3）的重要维护者。这些结果支持TCII与任何哺乳动物目都没有关联，并表明蝙蝠（主要是蝙蝠）可能是克氏锥虫TCII亚群的放大宿主。(c) 2008 Elsevier B.V.版权所有
Few studies have been conducted to investigate the role played by the order Chiroptera in the sylvatic transmission cycle of Trypanosoma cruzi or their putative association with the main genotypes of the parasite. Here, the purpose was to enlarge the knowledge of this issue, in this sense, 93 specimens of bats included in 4 families, respectively Molossidae, Noctilionidae, Phyllostomidae and Vespertilionidae collected in distinct regions of Brazil were submitted to fresh blood smears and hemocultures. No patent parasitemia was observed but positive hemocultures by T. cruzi were observed in 14% (13/93) of examined samples. The majority of the parasite isolates were obtained from Phyllostomus hastatus (80%) captured in one same buriti hollow palm tree in the Cerrado region. Multilocus enzyme electrophoresis (MLEE) analyses showed that the genetic distance among these isolates was 0.35, almost the same observed when all the isolates (excluding the reference strains) were analyzed (0.40). No correlation of zymodeme with bat genera, species or geographic region of its origin could be observed, moreover, correlation of zymodeme and genotype of the parasite was not strict. Ten out of 14 T. cruzi isolates obtained from bats corresponded to the TCII genotype. Chiropterans with TCI, TCII/TCIII mixed infection as well as Trypanosoma rangeli in single or mixed infections were observed. These results show that bats may harbor and are probably important maintainers of the main genotypes (TCI, TCII, TCII/Z3) of T. cruzi. These results support the absence of an association of TCII with any mammal order and show that bats, mainly P hastatus, may act as amplifier hosts of TCII subpopulations of T. cruzi. (c) 2008 Elsevier B.V. All rights reserved.