Effect of bleeding treatment and perfusion of yellowtail on lipid oxidation in post-mortem muscle
Effect of bleeding treatment and perfusion of yellowtail on lipid oxidation in post-mortem muscle
复制标题
鰤鱼放血和灌注对死后肌肉脂质氧化的影响
DOI：
10.1016/j.foodchem.2006.12.062
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发表时间：
2007
期刊：
影响因子：
8.8
通讯作者：
T. Ohshima
中科院分区：
文献类型：
--
作者：
J. Sohn;H. Ushio;N. Ishida;M. Yamashita;Makoto Terayama;T. Ohshima
本研究探讨了放血处理和灌注抗氧化剂对冰藏初期黄尾鱼普通肌肉和深色肌肉脂质氧化的影响。经放血处理和未经放血处理的黄尾鱼深色肌肉的脂质过氧化氢含量均高于普通肌肉，且上升速度更快。有和无出血治疗的深色肌肉之间的脂质过氧化氢含量（48小时），脂肪酸组成和高铁肌红蛋白形成的变化率没有显着差异。将含有抗坏血酸或6-羟基-2，5，7，8-四甲基苯并二氢吡喃-2-羧酸（Trolox®）的生理盐水灌注到活的黄尾鱼中或添加到切碎的深色肌肉中。在灌注实验中，与抗坏血酸相比，Trolox®显著（P<0.01）延迟了暗肌中脂质过氧化氢的积累。这些结果表明，简单地通过放血从活的黄尾鱼中去除一部分血液不足以防止冰储存早期阶段的脂质氧化。相反，在鱼肉中添加抗氧化剂可以有效地延缓死后肌肉中的脂质氧化。
The present study investigated the effects of bleeding treatment and perfusion of antioxidant compounds on lipid oxidation in ordinary and dark muscles of yellowtail in the early stage of ice storage. The lipid hydroperoxide contents of dark muscles obtained from yellowtails with and without bleeding treatment were higher and increased more rapidly than those of ordinary muscles. There were no significant differences in the rates of change of the lipid hydroperoxide content (up to 48h), fatty acid composition and metmyoglobin formation between dark muscles with and without bleeding treatment. Physiological saline containing ascorbic acid or 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox®) was perfused into live yellowtail or added to minced dark muscle. Trolox®significantly (P<0.01) delayed the accumulation of lipid hydroperoxide in dark muscle compared to ascorbic acid in perfusion experiment. These results indicate that simply removing a portion of the blood from live yellowtail by bleeding is not sufficient to prevent lipid oxidation in the early stage of ice storage. Contrary to this, addition of antioxidants into fish flesh is effective to delay lipid oxidation in post-mortem muscle.