Role of HA and BG in engineering poly(epsilon-caprolactone) porous scaffolds for accelerating cranial bone regeneration
Role of HA and BG in engineering poly(epsilon-caprolactone) porous scaffolds for accelerating cranial bone regeneration
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HA 和 BG 在工程聚（ε-己内酯）多孔支架中加速颅骨再生的作用
DOI：
10.1002/jbm.a.36584
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发表时间：
2019
影响因子：
4.9
通讯作者：
Li Zhong-Ming
中科院分区：
文献类型：
--
作者：
Yin Hua-Mo;Li Xiang;Wang Peng;Ren Yue;Liu Wei;Xu Jia-Zhuang;Li Ji-Hua;Li Zhong-Ming
不同的生物活性填料对多孔聚合物/无机复合支架生物行为的影响缺乏全面的比较，仍然难以捉摸。此外，高孔率的复合支架力学性能较差。采用高压成型法和盐析法制备了分别负载羟基磷灰石(HA)和生物活性玻璃(BG)的聚己内酯(ε)复合多孔支架。结构分析表明，所有多孔支架均呈连通的开孔结构，孔隙率为87%，孔径为180μm，与致孔剂的数量和大小有关。与PCL/HA支架相比，PCL/BG支架的吸水率提高了约2.3倍。由于骨架致密，PCL/HA和PCL/BG多孔支架具有优异的压缩弹性系数，显著高于文献报道的其他PCL复合多孔支架。细胞培养结果显示，PCL/BG支架成骨分化能力强于PCL和PCL/HA支架。活体实验结果显示，PCL/BG支架较PCL/HA支架有更多的成熟骨形成，说明BG的引入促进了颅骨的再生，在短时间内获得了完全的骨愈合。因此，这些数据表明PCL/BG支架在骨组织工程应用中更具竞争力。2018年Wiley期刊，Inc.生物材料资源A部分：107A：654-662,2019年。
Effects of varied bioactive fillers on the biological behavior of porous polymer/inorganic composite scaffolds are lack of comprehensive comparison and remain elusive. Moreover, composite scaffolds with high porosity suffer from inferior mechanical performance. Herein, high‐pressure molding and salt leaching were employed to prepare poly(ε‐caprolactone) (PCL) composite porous scaffolds loaded with hydroxyapatite (HA) and bioactive glass (BG), respectively. Structural analysis indicated all the porous scaffolds presented interconnected open‐pore structure with the porosity of ~87% and pore size of ~180 μm, hinging on the amounts and size of porogen. Compared to PCL/HA scaffolds, PCL/BG scaffolds showed ~2.3‐fold augment in the water absorption. Attributing to the compact framework, the PCL/HA and PCL/BG porous scaffolds exhibited outstanding compressive modulus, which was notably higher than other PCL composite porous scaffolds reported in literatures. Cells culture results demonstrated that PCL/BG scaffolds displayed higher expression of osteogenic differentiation than PCL and PCL/HA scaffolds. Furthermore,in vivoresults showed that more mature bone was formed within PCL/BG scaffolds than PCL/HA scaffolds, manifesting that the introduction of BG accelerated cranial bone regeneration to obtain complete bone healing within a short time. Therefore, these data indicate that PCL/BG scaffolds are more competitive for bone tissue engineering application. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 654–662, 2019.