Development of a High-speed Super-resolution Confocal Scanner
Development of a High-speed Super-resolution Confocal Scanner
复制标题
高速超分辨率共焦扫描仪的研制
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Takayuki Kei
中科院分区：
文献类型：
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作者：
T. Azuma;Takayuki Kei
超分辨率显微镜技术克服了传统光学显微镜的空间分辨率限制，已经被开发出来，并越来越多地用于包括细胞生物学在内的各个领域的高级研究。然而，它们的时间分辨率仍然很低，因此很难观察活细胞的动态行为。此外，这些现有技术对可使用的染料和可观察的样品具有限制。因此，越来越需要一种通用的超分辨率显微镜技术，可以可视化的活细胞的精细结构与高时间分辨率。为了满足这一需求，横河电机基于其共焦扫描单元（CSU）开发了一种高速超分辨率共焦扫描仪，该扫描仪被证明是观察活细胞的理想选择。本文概述了在这种新的扫描仪和它的应用程序中实现的技术。
Super-resolution microscopy techniques that overcome the spatial resolution limit of conventional light microscopy have been developed and are increasingly used for advanced research in various fields including cell biology. However, their temporal resolution is still low, so it is difficult to observe the dynamic behaviors of living cells. Furthermore, these existing techniques have restrictions on the dyes that can be used and the specimens that can be observed. Therefore, there is an increasing need for a versatile super-resolution microscopy technique that can visualize the fine structures of living cells with high temporal resolution. To meet this need, Yokogawa has developed a high-speed super-resolution confocal scanner based on its confocal scanner unit (CSU), which is proven to be ideal for observing living cells. This paper outlines the technologies implemented in this new scanner and its applications.