The Use of Cellular Magnetic Resonance Imaging to Track the Fate of Iron-Labeled Multipotent Stromal Cells after Direct Transplantation in a Mouse Model of Spinal Cord Injury
The Use of Cellular Magnetic Resonance Imaging to Track the Fate of Iron-Labeled Multipotent Stromal Cells after Direct Transplantation in a Mouse Model of Spinal Cord Injury
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DOI：
10.1007/s11307-010-0393-y
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发表时间：
2011-08-01
影响因子：
3.1
通讯作者：
Foster, Paula J.
中科院分区：
文献类型：
--
作者：
Gonzalez-Lara, Laura E.;Xu, Xiaoyun;Foster, Paula J.
本研究的目的是通过磁共振成像（MRI）追踪铁标记的多能基质细胞（MSC）直接移植到脊髓损伤小鼠体内后的命运。或（4）游离MPIO，并在移植后6周在3 T下成像。铁标记的活MSC在移植部位的小鼠脊髓中出现明确的信号丢失区域。然而，死亡的，铁标记的MSC和游离的铁颗粒的MR表现是相似的，并持续了6周的研究。铁标记的干细胞直接移植到小鼠损伤的脊髓后，可以在体内检测和监测。不过，铁标的命运并不明朗。我们的调查表明，应谨慎解释磁共振图像后，直接移植铁标记的细胞。
The objective of this study was to track the fate of iron-labeled, multipotent stromal cells (MSC) after their direct transplantation into mice with spinal cord injuries using magnetic resonance imaging (MRI).Mice with spinal cord injuries received a direct transplant of (1) live MSC labeled with micron-sized iron oxide particles (MPIO); (2) dead, MPIO-labeled MSC; (3) unlabeled MSC; or (4) free MPIO and were imaged at 3 T for 6 weeks after transplantation.Live, iron-labeled MSC appeared as a well-defined region of signal loss in the mouse spinal cord at the site of transplant. However, the MR appearance of dead, iron-labeled MSC and free iron particles was similar and persisted for the 6 weeks of the study.Iron-labeled stem cells can be detected and monitored in vivo after direct transplantation into the injured spinal cord of mice. However, the fate of the iron label is not clear. Our investigation indicates that caution should be taken when interpreting MR images after direct transplantation of iron-labeled cells.