Catecholamines are present in larval Xenopus laevis:: A potential source for cardiac control
Catecholamines are present in larval Xenopus laevis:: A potential source for cardiac control
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DOI：
10.1002/jez.10049
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发表时间：
2002-02-15
影响因子：
--
通讯作者：
Fritsche, R
中科院分区：
文献类型：
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作者：
Kloberg, AJ;Fritsche, R
采用高效液相色谱法（HPLC）研究了非洲爪蟾（Xenopus laevis）胚胎发育过程中心脏、肾脏和全身（不包括心脏和肾脏）去甲肾上腺素（NA）、肾上腺素（A）和多巴胺（DA）水平的变化。此外，存在的细胞免疫反应酪氨酸羟化酶（TH），多巴胺-β-羟化酶（DBH）和/或苯乙醇胺-N-甲基转移酶（PNMT）在爪蟾幼虫的心脏进行了研究，使用免疫组织化学技术。使用抗乙酰化微管蛋白（AcT）的抗体观察神经纤维的存在。心脏中NA和DA浓度在NF 40-56时较低且稳定，NF 57时显示出增加的值，并且在幼蛙中再次降低。在NF 43-49和NF 57处观察到A浓度升高的趋势。TH、DBH和PNMT免疫反应阳性细胞从NF 40开始在心脏中出现，到后期整个心脏中TH免疫反应阳性细胞增多。在非神经支配的幼虫心脏中的存在下，与TH/DBH/PNMT免疫反应细胞的发现表明，儿茶酚胺的合成和存储在心脏中，因此可能有一个旁分泌的作用，在爪蟾幼虫的心脏控制。在肾脏和全身（心脏和肾脏除外）中也发现了可检测浓度的儿茶酚胺。因此，心脏外的儿茶酚胺产生细胞可能是爪蟾幼虫肾上腺素能心脏控制中循环儿茶酚胺的重要来源。(C)2002 Wiley-Liss，Inc.
Changes in noradrenaline (NA), adrenaline (A), and dopamine (DA) levels in the heart, kidneys, and whole body (without heart and kidneys) during embryonic development were investigated in the frog, Xenopus laevis using high-performance liquid chromatography (HPLC). In addition, the presence of cells immunoreactive to tyrosine hydroxylase (TH), dopamine-beta-hydroxylase (DBH) and/or phenylethanolamine-N-methyltransferase (PNMT) in the heart of Xenopus larvae was investigated using immunohistochemical techniques. The presence of nerve fibers was visualized using antibodies against acetylated tubulin (AcT). NA and DA concentrations in the heart were low and steady in NF 40-56, showed an increased value at NF 57, and decreased again in froglets. A trend toward higher concentrations of A was observed at NF 43-49 and NF 57. Cells immunoreactive to TH, DBH, and PNMT were found in the heart from NF 40, and the TH immunoreactive cells became more abundant in the whole heart at later stages. The presence of catecholamines in the non-innervated larval heart together with the finding of TH/DBH/PNMT immunoreactive cells suggests that catecholamines are synthesized and stored in the heart and could therefore have a paracrine role in cardiac control in Xenopus larvae. Detectable concentrations of catecholamines were also found in kidneys and whole bodies (except heart and kidneys). Therefore, catecholamine-producing cells outside the heart can be an important source of circulating catecholamines involved in adrenergic cardiac control in Xenopus larvae. (C) 2002 Wiley-Liss, Inc.