Monitoring motility, spreading, and mortality of adherent insect cells using an impedance sensor
Monitoring motility, spreading, and mortality of adherent insect cells using an impedance sensor
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DOI：
10.1021/ac0011585
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发表时间：
2001-04-15
影响因子：
7.4
通讯作者：
Kamen, A
中科院分区：
文献类型：
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作者：
Luong, JHT;Habibi-Rezaei, M;Kamen, A
一种新兴的传感器技术被称为细胞-基质阻抗传感（ECIS），已经扩展到监测昆虫细胞的行为，包括附着、运动和死亡。在ECIS中，将贴壁细胞培养在放置在组织培养孔底部的8个小金电极阵列上，并浸入培养基中。随着细胞在金电极上的附着和扩散，阻抗增加，因为细胞作为绝缘颗粒来限制电流的流动。实验数据表明，昆虫细胞与不同的蛋白相互作用不同，这些蛋白被用来预先包裹在金电极上，豆豆蛋白A作为最佳的启动子，以加速细胞的附着速度。在细胞完全扩散后，测量的阻抗继续波动，以反映细胞的持续运动和代谢活性。由于细胞行为对外界化学物质敏感，ECIS用于抑制实验的适用性以HgCl2、三硝基甲苯、三硝基苯（TNB）和2-氨基-4,6-二硝基甲苯为模型体系。与传统分析不同，本研究中获得的定量数据是实时和连续的，以描述细胞运动和死亡率。
An emerging sensor technology referred to as electric cell-substrate impedance sensing (ECIS) has been extended for monitoring the behavior of insect cells including attachment, motility, and mortality. In ECIS, adherent cells were cultured on an array of eight small gold electrodes deposited on the bottom of tissue culture wells and immersed in a culture medium. Upon the attachment and spreading of cells on the gold electrode, the impedance increased because the cells acted as insulating particles to restrict the current flow. Experimental data revealed that insect cells interacted differently with various proteins used to precoat the gold electrode with concanavalin A as the best promoter to accelerate the rate of cell attachment. After the cells were fully spread, the measured impedance continued to fluctuate to reflect the constant motion and metabolic activity of the cells. As the cell behavior was sensitive to external chemicals, the applicability of ECIS for inhibition assays was demonstrated with HgCl2, trinitrotoluene, trinitrobenzene (TNB), and 2-amino-4,6-dinitrotoluene as model systems. Unlike conventional assays, the quantitative data obtained in this study are taken in real time and in a continuous fashion to depict cell motility and mortality.