Effects of 15-deoxyspergualin on experimental autoimmune giant cell myocarditis of the rat.
Effects of 15-deoxyspergualin on experimental autoimmune giant cell myocarditis of the rat.
复制标题
15-脱氧精胍菌素对大鼠实验性自身免疫性巨细胞心肌炎的影响。
DOI：
10.1161/01.cir.91.4.1116
复制
发表时间：
1995
期刊：
影响因子：
37.8
通讯作者：
A. Shibata
中科院分区：
文献类型：
--
作者：
M. Kodama;S. Zhang;H. Hanawa;M. Saeki;T. Inomata;K. Suzuki;S. Koyama;A. Shibata
背景
免疫抑制疗法对人类心肌炎的益处是有争议的。本文观察了新型免疫抑制剂15-脱氧精胍菌素（DSG）对实验性自身免疫性心肌炎（EAM）大鼠的作用。
方法和结果
在第0天用弗氏完全佐剂中的心肌肌球蛋白免疫刘易斯大鼠。在第一个实验中，研究了预防EAM所需的DSG的有效剂量。将大鼠分为5组：对照组（A）从第1天至第10天给予生理盐水; B组，0.3 mg/kg/天DSG; C组，1.0 mg/kg/天DSG; D组，3.0 mg/kg/天DSG; E组，10.0 mg/kg/天DSG。在第28天处死大鼠。D、E组大鼠心脏重量/体重比明显低于对照组。D组和E组心肌炎的肉眼和显微镜评分降低。在接下来的实验中，研究了DSG延迟给药在预防自身免疫性心肌炎中的作用。两组大鼠从第6天至第15天分别接受3.0和10.0 mg/kg/天的DSG。另外两组大鼠从第11天至第20天接受相同剂量的DSG。延迟DSG治疗未观察到预防作用。然后评价长期延迟启动治疗的效果。从第6天至第25天，大鼠每天给予10.0 mg/kg DSG。与对照组相比，经此处理的大鼠的心脏重量/体重比以及肉眼和显微镜评分显著降低。
结论
DSG对心肌肌球蛋白诱导的自身免疫性心肌炎有预防作用。
BACKGROUND
The benefits of immunosuppressive therapy for human myocarditis are controversial. The effects of a new immunosuppressant agent, 15-deoxyspergualin (DSG), on rats with experimental autoimmune myocarditis (EAM), an animal model of human giant cell myocarditis, were examined.
METHODS AND RESULTS
Lewis rats were immunized with cardiac myosin in Freund's complete adjuvant on day 0. In the first experiment, the effective doses of DSG required to prevent EAM were investigated. Rats were placed into one of five groups: the control group (A) was administered saline from days 1 to 10; group B, 0.3 mg/kg per day of DSG; group C, 1.0 mg/kg per day of DSG; group D, 3.0 mg/kg per day of DSG, and group E, 10.0 mg/kg per day of DSG. Rats were killed on day 28. The heart weight/body weight ratios of the rats of groups D and E were significantly lower than that of the control group. Macroscopic and microscopic scores for myocarditis decreased in groups D and E. In the next experiment, the effects of delayed administration of DSG in preventing autoimmune myocarditis were studied. Two groups of rats received 3.0 and 10.0 mg/kg per day of DSG from days 6 to 15, respectively. Two other groups of rats received the same doses of DSG from days 11 to 20. No preventive effect of delayed DSG treatment was observed. The effects of long-term, delayed initiation therapy then were evaluated. Rats were administered 10.0 mg/kg per day of DSG from days 6 to 25. The heart weight/body weight ratio and macroscopic and microscopic scores of the rats so treated significantly decreased compared with the controls.
CONCLUSIONS
It was demonstrated that DSG can prevent the development of cardiac myosin-induced autoimmune myocarditis.
影响因子：
24
作者：
A. Matsumori;C. Crumpacker;W. Abelmann
通讯作者：
A. Matsumori;C. Crumpacker;W. Abelmann
影响因子：
37.8
作者：
MATSUMORI, A;WANG, H;CRUMPACKER, CS
通讯作者：
CRUMPACKER, CS
环孢素治疗脑心肌炎病毒引起的实验性小鼠心肌炎。
影响因子：
37.8
作者：
Monrad,ES;Matsumori,A;Murphy,JC;Fox,JG;Crumpacker,CS;Abelmann,WH
通讯作者：
Abelmann,WH