TANDEM DUPLICATIONS RESULTING FROM RECOMBINATION BETWEEN RIBOSOMAL-RNA GENES IN ESCHERICHIA-COLI
TANDEM DUPLICATIONS RESULTING FROM RECOMBINATION BETWEEN RIBOSOMAL-RNA GENES IN ESCHERICHIA-COLI
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DOI：
10.1016/0022-2836(77)90077-8
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发表时间：
1977-01-01
影响因子：
5.6
通讯作者：
HILLMAN, BS
中科院分区：
文献类型：
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作者：
HILL, CW;GRAFSTROM, RH;HILLMAN, BS
一组E. coli K12突变体，其携带串联重复的glyT purD区域，进行了分析。三种类型的重复重复发生，它们是由位于glyT purD区域一侧的rRNA基因rrnE和在该区域另一侧直接重复的3个rrn基因中的1个之间的重组产生的。这些重复突变体的表征涉及以DNA环形式分离重复材料。从rrnB延伸到rrnE的I类重复长39，500个碱基对，从rrnA延伸到rrnE的II类重复长164，000个碱基对，从rrnC延伸到rrnE的III类重复长258，000个碱基对。
A set of E. coli K12 mutants, which carry a tandem duplication of the glyT purD region, were analyzed. Three types of duplications occurred repeatedly, and they were generated by recombination between the rRNA gene, rrnE, which lies to one side of the glyT purD region and 1 of 3 rrn genes which occur as direct repetitions on the other side of this region. Characterization of these duplication mutants has involved the isolation of the duplicated material in the form of a DNA circle. Class I duplications, which extend from rrnB to rrnE, are 39,500 base-pairs long, class II duplications, which extend from rrnA to rrnE, are 164,000 base-pairs long, and class III duplications, which extend from rrnC torrnE, are 258,000 base-pairs long.