PKCγ in Vc and C1/C2 is Involved in Trigeminal Neuropathic Pain
PKCγ in Vc and C1/C2 is Involved in Trigeminal Neuropathic Pain
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DOI：
10.1177/0022034511401406
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发表时间：
2011-06-01
影响因子：
7.6
通讯作者：
Iwata, K.
中科院分区：
文献类型：
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作者：
Nakajima, A.;Tsuboi, Y.;Iwata, K.
本研究的目的是阐明蛋白激酶 C γ (PKC γ) 在眶下神经损伤后面部神经病理性疼痛中的作用。我们分析了眶下神经慢性压迫性损伤（ION-CCI）后三叉神经脊髓尾亚核（Vc）和上颈脊髓（C1/C2）中 PKC γ 表达的变化。我们还研究了 ION-CCI 介导的大鼠机械伤害行为。与 ION-CCI 之前和假手术大鼠相比，ION-CCI 后 1 至 14 天机械退缩阈值显着降低。 ION-CCI术后第3、7和14天，ION-CCI大鼠同侧Vc中PKCγ的表达明显高于对侧。鞘内 (i.t.) 施用 PKC γ 抑制剂白屈菜红碱可防止同侧 Vc 中 PKC γ 表达的增加。此外，i.t.白屈菜红碱的施用消除了 ION-CCI 介导的退缩阈值的降低。综上所述，这些发现表明 Vc 中 PKC γ 的表达在三叉神经损伤后口面部静态机械性异常性疼痛的机制中发挥着重要作用。
The aim of the present study was to clarify the involvement of protein kinase C gamma (PKC gamma) in the facial neuropathic pain following infraorbital nerve injury. We analyzed the change in PKC gamma expression in the trigeminal spinal subnucleus caudalis (Vc) and upper cervical spinal cord (C1/C2) following chronic constriction injury of the infraorbital nerve (ION-CCI). We also studied ION-CCI-mediated mechanical nocifensive behavior in rats. The mechanical head-withdrawal threshold significantly decreased 1 to 14 days after ION-CCI compared with that before ION-CCI and in sham rats. The expression of PKC gamma was significantly larger in the ipsilateral Vc compared with the contralateral side in ION-CCI rats 3, 7, and 14 days after ION-CCI. Intrathecal (i.t.) administration of the PKC gamma inhibitor chelerythrine prevented an increase in the PKC gamma expression in the ipsilateral Vc. Moreover, i.t. administration of chelerythrine annulled ION-CCI-mediated reduction in the head-withdrawal threshold. Taken together, these findings suggest that PKC gamma expression in the Vc played an important role in the mechanism of orofacial static mechanical allodynia following trigeminal nerve injury.