Stiffness of Cargo-Motor Linkage Tunes Myosin VI Motility and Response to Load.
Stiffness of Cargo-Motor Linkage Tunes Myosin VI Motility and Response to Load.
复制标题
货物-电机联动装置的刚度可调节肌球蛋白 VI 的运动性和对负载的响应。
DOI：
10.1021/acs.biochem.9b00422
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发表时间：
2019
期刊：
影响因子：
2.9
通讯作者：
Sivaramakrishnan,Sivaraj
中科院分区：
文献类型：
--
作者：
Shrivastava,Rachit;Rai,Ashim;Salapaka,Murti;Sivaramakrishnan,Sivaraj
We examine the effect of cargo–motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo–motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiffness on the motile properties of myosin VI. Our results reveal that a stiff cargo–motor linkage leads to shorter step sizes and load-induced anchoring of myosin VI, while a flexible linkage results in longer steps with frequent detachments from the actin filament under load. Our findings suggest a novel regulatory mechanism for tuning the dual cellular roles of the anchor and transporter ascribed to myosin VI.
