Genome-Wide Identification of Jatropha curcas Aquaporin Genes and the Comparative Analysis Provides Insights into the Gene Family Expansion and Evolution in Hevea brasiliensis.
Genome-Wide Identification of Jatropha curcas Aquaporin Genes and the Comparative Analysis Provides Insights into the Gene Family Expansion and Evolution in Hevea brasiliensis.
复制标题
麻风树水通道蛋白基因的全基因组鉴定和比较分析为巴西橡胶树基因家族的扩展和进化提供了见解
DOI：
10.3389/fpls.2016.00395
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发表时间：
2016
影响因子：
5.6
通讯作者：
Xie G
中科院分区：
文献类型：
--
作者：
Zou Z;Yang L;Gong J;Mo Y;Wang J;Cao J;An F;Xie G
水通道蛋白（AQP）是一种能将水和其他小分子溶质转运到生物膜上的通道蛋白。尽管AQP的重要作用，但迄今为止，对麻风树（Jatropha curcas L.，大戟科（Euphorbiaceae）是一种重要的非食用油料作物，具有生产生物柴油的巨大潜力。在这项研究中，32个AQP基因被鉴定出的坚果基因组和家庭的数量相对较小的相比，在另一个大戟科植物，橡胶树（橡胶树）的51。Arg.）。基于系统发育分析，将JcAQP分为5个亚家族，即，9种质膜内在蛋白（PIP）、9种液泡膜内在蛋白（TIP）、8种NOD 26样内在蛋白（NIP）、2种X内在蛋白（XIP）和4种小碱性内在蛋白（SIP）。像橡胶树和其他植物物种，功能预测的基础上的芳香族/精氨酸选择性过滤器，Froger的位置，和特异性决定的位置表现出显着的差异JcAQP的亚家族之间的底物特异性。全基因组比较分析表明PIP和TIP亚家族在橡胶树中特异性扩增，而XIP亚家族在核桃中特异性缺失。此外，通过对深度转录组测序数据的分析，对重复HbAQP基因的表达进化，特别是表达差异进行了研究和讨论。本研究结果不仅为Jc/HbAQP基因的功能分析和利用提供了有价值的信息，也为进一步研究大戟科植物及其他植物中Jc/HbAQP基因家族的扩展和进化提供了参考。
Aquaporins (AQPs) are channel-forming integral membrane proteins that transport water and other small solutes across biological membranes. Despite the vital role of AQPs, to date, little is known in physic nut (Jatropha curcas L., Euphorbiaceae), an important non-edible oilseed crop with great potential for the production of biodiesel. In this study, 32 AQP genes were identified from the physic nut genome and the family number is relatively small in comparison to 51 in another Euphorbiaceae plant, rubber tree (Hevea brasiliensis Muell. Arg.). Based on the phylogenetic analysis, the JcAQPs were assigned to five subfamilies, i.e., nine plasma membrane intrinsic proteins (PIPs), nine tonoplast intrinsic proteins (TIPs), eight NOD26-like intrinsic proteins (NIPs), two X intrinsic proteins (XIPs), and four small basic intrinsic proteins (SIPs). Like rubber tree and other plant species, functional prediction based on the aromatic/arginine selectivity filter, Froger's positions, and specificity-determining positions showed a remarkable difference in substrate specificity among subfamilies of JcAQPs. Genome-wide comparative analysis revealed the specific expansion of PIP and TIP subfamilies in rubber tree and the specific gene loss of the XIP subfamily in physic nut. Furthermore, by analyzing deep transcriptome sequencing data, the expression evolution especially the expression divergence of duplicated HbAQP genes was also investigated and discussed. Results obtained from this study not only provide valuable information for future functional analysis and utilization of Jc/HbAQP genes, but also provide a useful reference to survey the gene family expansion and evolution in Euphorbiaceae plants and other plant species.