Cytotoxic T lymphocyte mediated lysis without release of serine esterase
Cytotoxic T lymphocyte mediated lysis without release of serine esterase
复制标题
细胞毒性 T 淋巴细胞介导的裂解而不释放丝氨酸酯酶
作者：
H. Ostergaard;K. Kane;M. Mescher;W. Clark
克隆的细胞毒性T淋巴细胞（CTL）可以以Ca2+依赖或Ca2+独立的方式裂解靶细胞，这取决于所使用的靶细胞1,2。我们已经使用这些无Ca2+的测定条件来确定在没有细胞外Ca2+的情况下是否有丝氨酸酯酶释放。我们发现，在没有Ca2+的情况下，有显著的靶细胞杀伤的条件下，没有可检测到的丝氨酸酯酶释放。在一定程度上，丝氨酸酯酶和穿孔蛋白样分子存在于相同的颗粒中，这些结果也表明，在没有脱粒的情况下，靶细胞可能会被杀死。我们的结果提供了一个没有丝氨酸酯酶释放的靶细胞杀死的例子，并且首次表明裂解和脱粒可以在功能上解离。
Cloned cytotoxic T lymphocytes (CTL) can lyse target cells in a Ca2+-dependent or Ca2+-independent fashion, depending on the target cell used1,2 We have used these Ca2+-free assay conditions to determine whether there is serine esterase release in the absence of extracellular Ca2+. We find that under conditions where there is significant target cell killing in the absence of Ca2+, there is no detectable serine esterase release. To the extent that serine esterase and perforin-like molecules reside in the same granules, these results also suggest that target cell killing may occur in the absence of degranulation. Our results provide an example of target cell killing without serine esterase release, and the first indication that lysis and degranulation can be functionally dissociated.