Viral Diversity of House Mice in New York City.
Viral Diversity of House Mice in New York City.
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DOI：
10.1128/mbio.01354-17
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发表时间：
2018-04-17
期刊：
影响因子：
6.4
通讯作者：
Lipkin WI
中科院分区：
文献类型：
--
作者：
Williams SH;Che X;Garcia JA;Klena JD;Lee B;Muller D;Ulrich W;Corrigan RM;Nichol S;Jain K;Lipkin WI
野生小家鼠（家鼠）是一种全球分布的入侵性害虫，与城市中心的人类密切接触，其微生物组在很大程度上尚未被探索。在这里，我们报告了纽约市住宅楼家鼠粪便病毒组的分析。在为期1年的时间内，在曼哈顿、皇后区、布鲁克林和布朗克斯的7个地点收集小鼠。粪便无偏高通量测序结果显示，36种病毒来自18科21属，其中至少包括6种新病毒和3种新属。通过PCR对15种病毒进行的代表性筛选证实了肝脏中存在这些病毒中的13种。我们发现了多样性的不均匀分布，有几种病毒与特定的位置有关。在调整部位、性别和长度后，小鼠体重增加与每只小鼠检测到的病毒数量增加相关。我们既没有发现已知人类病毒病原体的遗传足迹，也没有发现淋巴细胞性脉络丛脑膜炎病毒的抗体。小鼠携带多种具有人畜共患潜力的传染因子。因此，它们在建筑环境中接近人类是一个公共卫生问题。实验室小鼠也是研究传染病病理生物学的最常见的实验模型。在这项对纽约市内多个地点捕获的小鼠进行的为期1年的调查中，我们发现了多种病毒，其中包括一些以前与家鼠无关的病毒和其他似乎是新的病毒。虽然我们没有发现已知的人类病原体，但我们的研究结果提供了对病毒生态学的见解，并可能产生对临床微生物学有用的模型。
The microbiome of wild Mus musculus (house mouse), a globally distributed invasive pest that resides in close contact with humans in urban centers, is largely unexplored. Here, we report analysis of the fecal virome of house mice in residential buildings in New York City, NY. Mice were collected at seven sites in Manhattan, Queens, Brooklyn, and the Bronx over a period of 1 year. Unbiased high-throughput sequencing of feces revealed 36 viruses from 18 families and 21 genera, including at least 6 novel viruses and 3 novel genera. A representative screen of 15 viruses by PCR confirmed the presence of 13 of these viruses in liver. We identified an uneven distribution of diversity, with several viruses being associated with specific locations. Higher mouse weight was associated with an increase in the number of viruses detected per mouse, after adjusting for site, sex, and length. We found neither genetic footprints to known human viral pathogens nor antibodies to lymphocytic choriomeningitis virus. Mice carry a wide range of infectious agents with zoonotic potential. Their proximity to humans in the built environment is therefore a concern for public health. Laboratory mice are also the most common experimental model for investigating the pathobiology of infectious diseases. In this survey of mice trapped in multiple locations within New York City over a period of 1 year, we found a diverse collection of viruses that includes some previously not associated with house mice and others that appear to be novel. Although we found no known human pathogens, our findings provide insights into viral ecology and may yield models that have utility for clinical microbiology.
影响因子：
11.8
作者：
Asnis DS;Muana O;Kim DG;Garcia M;Rollin PE;Slavinski S
通讯作者：
Slavinski S