Human leukocyte elastase, cathepsin G, and lactoferrin: family of neutrophil granule glycoproteins that bind to an alveolar macrophage receptor.
Human leukocyte elastase, cathepsin G, and lactoferrin: family of neutrophil granule glycoproteins that bind to an alveolar macrophage receptor.
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人白细胞弹性蛋白酶、组织蛋白酶 G 和乳铁蛋白：与肺泡巨噬细胞受体结合的中性粒细胞颗粒糖蛋白家族。
DOI：
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发表时间：
1982
影响因子：
11.1
通讯作者：
E. Campbell
中科院分区：
文献类型：
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作者：
E. Campbell
多形核中性粒细胞和单核吞噬细胞之间的相互作用在多种炎症过程中具有潜在的重要意义。作为阐明人类肺泡巨噬细胞受体介导的中性粒细胞（白细胞）弹性酶结合的生理学重要性的持续努力的一部分，我研究了白细胞弹性酶和另外两种中性粒细胞颗粒糖蛋白（组织蛋白酶G和乳铁蛋白）与人类肺泡巨噬细胞的结合。所有三种配体在0℃下均可饱和结合，平衡解离常数分别为4.0 × 10(-7), 2.0 × 10（-7）和1.7 × 10(-6) M。每个细胞都绑定到相似的数量（54-73 x 10(6)）个位点。这三种配体的结合均被岩藻糖苷多糖所抑制，并且它们与巨噬细胞的结合被广泛的交叉抑制。结果表明，肺泡巨噬细胞对中性粒细胞颗粒糖蛋白家族具有相对低亲和力，高容量的受体，这将非常适合清除炎症组织中细胞外间隙释放的中性粒细胞颗粒内容物。
Interactions between polymorphonuclear neutrophils and mononuclear phagocytes are potentially of great importance in a variety of inflammatory processes. As part of a continuing effort to elucidate the physiologic importance of human alveolar macrophage receptor-mediated binding of neutrophil (leukocyte) elastase, I have studied the binding of leukocyte elastase and two other neutrophil granule glycoproteins, cathepsin G and lactoferrin, to human alveolar macrophages. Saturable binding of all three ligands at 0 degrees C was observed, with equilibrium dissociation constants of 4.0 x 10(-7), 2.0 x 10(-7), and 1.7 x 10(-6) M, respectively. All bound to a similar number (54-73 x 10(6)) of sites per cell. Binding of all three ligands was inhibited by the polysaccharide fucoidin, and extensive cross-inhibition of their binding to macrophages was observed. The results indicate that alveolar macrophages possess a relatively low-affinity, high-volume receptor for a family of neutrophil granule glycoproteins, which would be ideally suited for clearing released neutrophil granule contents from the extracellular space in inflamed tissues.