TURNOVER OF PROTEIN IN GROWING AND NON-GROWING POPULATIONS OF ESCHERICHIA-COLI
TURNOVER OF PROTEIN IN GROWING AND NON-GROWING POPULATIONS OF ESCHERICHIA-COLI
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DOI：
10.1042/bj0690110
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发表时间：
1958-01-01
影响因子：
4.1
通讯作者：
MANDELSTAM, J
中科院分区：
文献类型：
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作者：
MANDELSTAM, J
描述了估计E.大肠杆菌中通过使用具有特定氨基酸需求的菌株。在快速生长的细胞中，没有可检测到的蛋白质周转;在非生长细胞中，蛋白质的降解和再合成以4 - 5%/小时的速率进行数小时。氯霉素，叠氮化物和铵盐抑制蛋白质分解后的潜伏期。2，4-二硝基苯酚的作用是可变的。蛋白质周转不能归因于细胞裂解。讨论了蛋白质周转在细菌代谢中的可能作用。
Methods are described for the estimation of protein turnover in E. coli by the use of strains with specific amino acid requirements. In rapidly growing cells there was no detectable turnover of protein; in non-growing cells degradation and resynthesis of protein proceeded at the rate of 4 to 5%/hour for several hours. Chloramphenicol, azide and ammonium salts inhibited protein breakdown after a latent period. The effect of 2,4-dinitrophenol was variable. Protein turnover could not be attributed to lysis of cells. The possible function of protein turnover in bacterial metabolism is discussed.