Enantiomeric and Isomeric Separation of Pesticides by Cyclodextrin-Modified Micellar Electrokinetic Chromatography
Enantiomeric and Isomeric Separation of Pesticides by Cyclodextrin-Modified Micellar Electrokinetic Chromatography
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DOI：
10.1093/jaoac/82.6.1550
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发表时间：
1999-11
影响因子：
1.6
通讯作者：
D. Shea;Kumar V. Penmetsa;R. Leidy
中科院分区：
文献类型：
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作者：
D. Shea;Kumar V. Penmetsa;R. Leidy
应用环糊精修饰胶束电动色谱成功地分离了7种常用农药（丙环唑、生物丙烯菊酯、甲氰菊酯、苯醚菊酯、联苯三唑醇、三唑醇和烯酰吗啉）的对映体和异构体。评价了市售表面活性剂单独使用或与环糊精组合使用时对7种农药对映体/异构体的分离效果。本研究中使用的农药的对映异构体/异构体的基线分辨率>1.5。发现分辨率取决于运行缓冲液中所用的表面活性剂和环糊精的类型。在丙环唑和烯酰吗啉的情况下，通过将胆酸钠（手性表面活性剂）单独添加到运行缓冲液中也实现了基线分辨率。强化水样品的分析产生的回收率范围从45%至89%，方法检测限范围从0.18至2.1 ppb。已报道的毛细管电泳方法简便、快速、重现性好。
Cyclodextrin-modified micellar electrokinetic chromatography was applied successfully to the enantiomeric and isomeric separation of 7 commonly used pesticides (propiconazole, bioallethrin, fenpropathrin, phenothrin, bitertanol, triadimenol, and dimethomorph). Commercially available surfactants used alone or in combination with cyclodextrins were evaluated for separation of the enantiomers/isomers of the 7 pesticides. The enantiomers/isomers of the pesticides used in this study were baseline resolved with resolutions of >1.5. The resolution was found to depend on the type of surfactant and cyclodextrin employed in the running buffer. In the case of propiconazole and dimethomorph, baseline resolution was also achieved by adding sodium cholate (a chiral surfactant) alone to the running buffer. Analysis of fortified water samples yielded recoveries ranging from 45 to 89% and method detection limits ranging from 0.18 to 2.1 ppb. The reported capillary electrophoresis methods are simple, rapid, and reproducible.