AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis
AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis
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DOI：
10.1007/s00425-010-1122-0
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发表时间：
2010-03
期刊：
影响因子：
4.3
通讯作者：
Jing Xu;Yong-Sheng Tian;R. Peng;A. Xiong;B. Zhu;X. Jin;F. Gao;X. Fu;X. Hou;Q. Yao
中科院分区：
文献类型：
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作者：
Jing Xu;Yong-Sheng Tian;R. Peng;A. Xiong;B. Zhu;X. Jin;F. Gao;X. Fu;X. Hou;Q. Yao
钙依赖性蛋白激酶 (CDPK) 家族在植物信号转导过程中的各种生理途径中发挥着重要作用。拟南芥AtCPK6基因属于应激诱导型CDPKs的亚类，它受到盐和渗透应激的刺激。为了阐明AtCPK6的生理功能，获得了双CaMV 35S启动子控制下的转基因拟南芥植物。AtCPK6过表达植物表现出增强的盐/干旱胁迫耐受性。通过脯氨酸和丙二醛(MDA)的变化证实AtCPK6过表达植物的耐受性升高。实时PCR分析表明，AtCPK6过表达植物中几个胁迫调节基因的表达水平发生了改变。然而cpk6突变体与对照没有表现出明显差异。这些结果可能表明AtCPK6在功能上是多余的，并且是参与拟南芥耐盐/干旱胁迫的正调节因子。
The calcium-dependent protein kinase (CDPK) family is needed in plant signaling during various physiological pathways. TheArabidopsisAtCPK6gene belongs to the subclass of stress-inducible CDPKs, which is stimulated by salt and osmotic stress. To elucidate the physiological function ofAtCPK6, transgenicArabidopsisplants under the control of double CaMV 35S promoter were obtained.AtCPK6over-expressing plants showed enhanced tolerance to salt/drought stresses. The elevated tolerance of theAtCPK6over-expressing plants was confirmed by the change of proline and malondialdehyde (MDA). Real-time PCR analyses revealed that the expression levels of several stress-regulated genes were altered inAtCPK6over-expressing plants. However,cpk6mutant displayed no obvious difference with control. These results are likely to indicate that AtCPK6 is functionally redundant and a positive regulator involved in the tolerance to salt/drought stress inArabidopsis.