TamiR159 directed wheat TaGAMYB cleavage and its involvement in anther development and heat response.
TamiR159 directed wheat TaGAMYB cleavage and its involvement in anther development and heat response.
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TamIR159 定向小麦 TaGAMYB 裂解及其参与花药发育和热响应
DOI：
10.1371/journal.pone.0048445
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yao Y
中科院分区：
文献类型：
--
作者：
Wang Y;Sun F;Cao H;Peng H;Ni Z;Sun Q;Yao Y
在拟南芥和水稻中，miR 159调控的GAMYB样家族转录因子在花发育和谷物糊粉细胞中的赤霉素（GA）信号传导中起作用。在这项研究中，miR 159参与其推定的目标TaGAMYB的调节及其与小麦发育的关系进行了研究。首先，我们使用5′-RACE和瞬时表达系统证明了TaGAMYB 1和TaGAMYB 2的切割由miR 159指导。其次，我们在转基因水稻中过量表达TamiR 159、TaGAMYB 1和mTaGAMYB 1（miR 159结合位点受损），揭示了来自小麦前体的成熟miR 159在水稻中的积累导致抽穗期延迟和雄性不育。此外，在水稻中过表达mTaGAMYB 1的分蘖和一级分枝的数量相对于野生型增加。我们先前的研究报告，TamiR 159在小麦（Triticum aestivum L.）热胁迫处理2小时后下调。最值得注意的是，TamiR 159过表达水稻品系相对于野生型对热胁迫更敏感，表明热胁迫后小麦中TamiR 159的下调可能参与热胁迫相关的信号传导途径，进而有助于热胁迫耐受性。
In Arabidopsis and rice, miR159-regulated GAMYB-like family transcription factors function in flower development and gibberellin (GA) signaling in cereal aleurone cells. In this study, the involvement of miR159 in the regulation of its putative target TaGAMYB and its relationship to wheat development were investigated. First, we demonstrated that cleavage of TaGAMYB1 and TaGAMYB2 was directed by miR159 using 5′-RACE and a transient expression system. Second, we overexpressed TamiR159, TaGAMYB1 and mTaGAMYB1 (impaired in the miR159 binding site) in transgenic rice, revealing that the accumulation in rice of mature miR159 derived from the precursor of wheat resulted in delayed heading time and male sterility. In addition, the number of tillers and primary branches in rice overexpressing mTaGAMYB1 increased relative to the wild type. Our previous study reported that TamiR159 was downregulated after two hours of heat stress treatment in wheat (Triticum aestivum L.). Most notably, the TamiR159 overexpression rice lines were more sensitive to heat stress relative to the wild type, indicating that the downregulation of TamiR159 in wheat after heat stress might participate in a heat stress-related signaling pathway, in turn contributing to heat stress tolerance.
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影响因子：
11.6
作者：
GUBLER, F;KALLA, R;JACOBSEN, JV
通讯作者：
JACOBSEN, JV
影响因子：
7.4
作者：
Gocal, GFW;Sheldon, CC;King, RW
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作者：
Alonso-Peral, Maria M.;Li, Junyan;Millar, Anthony A.
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作者：
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