Direct neurite-osteoblastic cell communication, as demonstrated by use of an in vitro co-culture system
Direct neurite-osteoblastic cell communication, as demonstrated by use of an in vitro co-culture system
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DOI：
10.1016/j.febslet.2007.11.065
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发表时间：
2007-12-22
期刊：
影响因子：
3.5
通讯作者：
Togari, Akifumi
中科院分区：
文献类型：
--
作者：
Obata, Koji;Furuno, Tadahide;Togari, Akifumi
使用体外共培养的方法，包括培养的小鼠上级颈神经节和MC 3 T3-E1成骨细胞样细胞，我们发现，除了蝎毒（SV）引起神经突起激活通过细胞内Ca 2+动员，并在一个滞后期后，成骨细胞Ca 2+动员。SV没有任何直接影响成骨细胞的神经突起的情况下。α（1）-肾上腺素能受体（AR）拮抗剂哌唑嗪的加入剂量依赖性地阻止了SV神经激活导致的成骨细胞激活。这些结果表明，成骨细胞的活化是对神经元活化的直接反应，而神经元活化是由成骨细胞中的α（1）-AR介导的。(c)2007年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Using an in vitro co-culture approach comprising cultured murine superior cervical ganglia and MC3T3-E1 osteoblast-like cells, we found that the addition of scorpion venom (SV) elicited neurite activation via intracellular Ca2+ mobilization and, after a lag period, osteoblastic Ca2+ mobilization. SV did not have any direct effect on the osteoblastic cells in the absence of neurites. The addition of an alpha(1)- adrenergic receptor (AR) antagonist, prazosin, dose-dependently prevented the osteoblastic activation that resulted as a consequence of neural activation by SV. These results demonstrate that osteoblastic activation occurred as a direct response to neuronal activation, which activation was mediated by alpha(1)-ARs in the osteoblastic cells. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.