Burkholderia fungorum sp nov, and Burkholderia caledonica sp nov., two new species isolated from the environment, animals and human clinical samples
Burkholderia fungorum sp nov, and Burkholderia caledonica sp nov., two new species isolated from the environment, animals and human clinical samples
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DOI：
10.1099/00207713-51-3-1099
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发表时间：
2001-05-01
影响因子：
2.8
通讯作者：
Vandamme, P
中科院分区：
文献类型：
--
作者：
Coenye, T;Laevens, S;Vandamme, P
对从环境、动物和人类临床样品中分离的16株菌株进行了多相分类研究，包括DNA-DNA杂交、DNA碱基比测定、16 S rDNA序列分析、全细胞蛋白和脂肪酸分析以及广泛的生化表征。分离物属于伯克霍尔德氏菌属，与Burkholderia graminis、Burkholderia caribensis和Burkholderia phenazinium亲缘关系较近，G+C含量在61.9 ~ 62.2mol%之间。从根际分离的7个菌株被指定为Burkholderia caledonica sp. nov. [模式菌株LMC 19076（T）（= CCUG 422363（T））]。从环境、动物和人类临床样本中分离的9株菌株被指定为真菌伯克霍尔德菌新种[模式菌株LMG 16225（T）（= CCUC 31961（T））]。B的鉴别试验。graminis，B. B. caribensis，B.吩嗪鎓，B. caledonica和B. fungorum包括以下内容：海藻糖、柠檬酸盐、DL-正亮氨酸、己二酸盐和蔗糖的同化;硝酸盐还原;在0.5%NaCl存在下的生长;和β-半乳糖苷酶活性。
A polyphasic taxonomic study that included DNA-DNA hybridizations, DNA base ratio determinations, 16S rDNA sequence analyses, whole-cell protein and fatty acid analyses and an extensive biochemical characterization was performed on 16 strains isolated from the environment, animals and human clinical samples. The isolates belonged to the genus Burkholderia, were phylogenetically closely related to Burkholderia graminis, Burkholderia caribensis and Burkholderia phenazinium and had G+C contents between 61.9 and 62.2 mol%. Seven strains isolated from the rhizosphere were assigned to Burkholderia caledonica sp. nov. [type strain LMC 19076(T) (= CCUG 422363(T))]. Nine strains isolated from the environment, animals and human clinical samples were assigned to Burkholderia fungorum sp. nov. [type strain LMG 16225(T) (= CCUC 31961(T))]. Differential tests for B. graminis, B. caribensis, B. phenazinium, B. caledonica and B. fungorum include the following: assimilation of trehalose, citrate, DL-norleucine, adipate and sucrose; nitrate reduction; growth in the presence of 0.5 % NaCl; and beta -galactosidase activity.