Natural killer (NK) cell activity in the rat. I. Isolation and characterization of the effector cells.
Natural killer (NK) cell activity in the rat. I. Isolation and characterization of the effector cells.
复制标题
大鼠自然杀伤 (NK) 细胞活性。
作者：
Craig W. Reynolds;T. Timonen;R. Herberman
这项研究已经证明了大颗粒淋巴细胞（LGL）在大鼠中的存在，并暗示了这些细胞在介导该物种的自然杀伤（NK）细胞活性中的核心作用。各器官的LGL频率显示为：外周血=肺大于脾大于腹膜渗出液大于淋巴结。在胸腺或骨髓中发现很少或没有LGL。这种模式类似于NK活性的分布，除了肺，没有检测到活性。这些研究还表明，不连续的Percoll密度梯度可用于高度富集或耗尽脾脏和外周血中的LGL。富集的LGL群体显示出选择性结合NK敏感靶细胞，并显示出非常高的NK细胞溶解。由于人类NK活性也被发现与LGL明显相关，本研究结果表明，大鼠可能提供了一个非常有用的动物模型，详细研究的个体发育，调节，和体内相关的NK细胞。
This study has demonstrated the presence of large granular lymphocytes (LGL) in the rat and implicated a central role for these cells in mediating natural killer (NK) cell activity in this species. The LGL frequency from various organs was shown to be: peripheral blood = lung greater than spleen greater than peritoneal exudate greater than lymph node. Little or no LGL were found in the thymus or bone marrow. This pattern was similar to the distribution of NK activity, except for the lungs, which had no detectable activity. These studies have also shown that discontinuous Percoll density gradients can be used to highly enrich or deplete LGL from both spleen and peripheral blood. Enriched LGL populations were shown to bind selectively to NK-susceptible target cells and to demonstrate very high NK cell cytolysis. Since human NK activity has also been found to be clearly associated with LGL, the present findings indicate that rats may provide a very useful animal model for detailed studies of the ontogeny, regulation, and in vivo relevance of NK cells.