Rapid quantitation of lipid in microalgae by time-domain nuclear magnetic resonance
Rapid quantitation of lipid in microalgae by time-domain nuclear magnetic resonance
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DOI：
10.1016/j.mimet.2008.07.019
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发表时间：
2008-12-01
影响因子：
2.2
通讯作者：
Wu, Qingyu
中科院分区：
文献类型：
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作者：
Gao, Chunfang;Xiong, Wei;Wu, Qingyu
对一株小球藻进行了异养发酵，以提高细胞生长速率和油脂含量，生产生物柴油。为了优化微藻生物柴油的发酵过程，降低生产成本，快速测定微藻细胞中的脂质含量是至关重要的。采用尼罗红（NR）染色和时域核磁共振（TD-NMR）技术对原壳类栉孔扇贝的脂质含量进行了定量分析。这两种方法都是可行的，比常用的重量法更简单。TD-NMR法与脂质提取实验测定值的一致性（R-2 = 0.9973）优于NR染色法（R-2 = 0.9067）。此外，样本的较小标准差（
A specific strain of Chlorella protothecoides has been studied in heterotrophic fermentation for increasing cell growth rate and lipid content for biodiesel production. For optimizing the process of fermentation to reduce costs of alga-based biodiesel production, rapid determination of lipid content in microalgal cells is critical. Nile Red (NR) staining and time-domain nuclear magnetic resonance (TD-NMR) have been investigated to quantitate the lipid content in C protothecoides. Both methods were found feasible and simpler than gravimetric methods that are commonly employed. The TD-NMR method showed better agreement (R-2 = 0.9973) with the measured values from lipid extraction experiments than the NR staining method (R-2 = 0.9067). Additionally, the smaller standard deviations of the samples (