Intermittent Watt-level Ultrasonication Facilitates Vancomycin Release From Therapeutic Acrylic Bone Cement
Intermittent Watt-level Ultrasonication Facilitates Vancomycin Release From Therapeutic Acrylic Bone Cement
复制标题
间歇性瓦特级超声波促进万古霉素从治疗性丙烯酸骨水泥中释放。
DOI：
10.1002/jbm.b.31288
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发表时间：
2009-07-01
影响因子：
3.4
通讯作者：
Xu, Jia
中科院分区：
文献类型：
--
作者：
Cai, Xun-Zi;Chen, Xian-Zhen;Xu, Jia
超声有望提高万古霉素从骨水泥中的释放，尽管当局部药物水平超过最低抑制浓度（T（>MIC））时的时间长度没有被先前的毫瓦级超声处理方案延长。在40 ml磷酸盐缓冲液（PBS）中浸泡28 d的同时，对万古霉素骨水泥进行连续功率级超声处理（CUG）、间歇功率级超声处理（IUG）和不超声处理（NUG）。IUG的T（>MIC）是NUG的3倍以上。相反，CUG的T（>MIC）略有缩短。IUG在15 ~ 28 d的万古霉素亚治疗释放量是NUG的1/9。拟合方程表明，超声处理能显著促进IUG的突释和缓释，但持续超声处理阻碍了IUG的缓释。SEM图像显示，与CUG和NUG相比，IUG标本中的火山口和孔隙更密集，直径更大，残留药物更少。与连续超声相比，间歇瓦级超声可延长T（>MIC）并抑制万古霉素的亚治疗释放，从而改善超声促进万古霉素从骨水泥中的释放。这些机制可能与声学微流的分离力和推力的独特作用有关。
Ultrasound holds promise for enhancing the vancomycin release from cement though the length of time when local drug level exceeded the minimum inhibitory concentration (T(>MIC)) was not prolonged by the previous protocol of milliwatt-level ultrasonication. Here vancomycin-loaded cements were subjected to continuous watt-level ultrasonication (CUG), intermittent watt-level ultrasonication (IUG) or no ultrasonication (NUG) for 14 d during immersion in 40-ml phosphate buffered saline (PBS) for 28 d. The T(>MIC) for IUG was more than three times that for NUG. In contrast, T(>MIC) for CUG was slightly shortened. The subtherapeutic release of vancomycin between 15 d and 28 d for IUG was one-ninth that for NUG. The fitting equations indicated a significant enhancement on the burst release and the slow release for IUG; however, the continuous ultrasonication hampered the slow release. SEM images exhibited denser craters and pores with larger diameters and less residual drug in specimens from IUG relative to those from both CUG and NUG. Intermittent watt-level ultrasonication improved the ultrasound-enhanced vancomycin release from cement in view of the prolonged T(>MIC) and the inhibited subtherapeutic release compared with continuous ultrasonication. The mechanisms may be associated with the distinctive effects of detaching forces and pushing forces by acoustic microstreams.