Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ
Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ
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DOI：
10.1152/ajplung.2000.278.6.l1186
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发表时间：
2000-06-01
影响因子：
4.9
通讯作者：
Zegarra-Moran, O
中科院分区：
文献类型：
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作者：
Galietta, LJV;Folli, C;Zegarra-Moran, O
用干扰素-γ或肿瘤坏死因子-α处理体外培养的人支气管上皮细胞，以评估其对跨上皮离子转运的影响。短路电流测量显示，干扰素-γ(10-1000U/ml)对Na+吸收有明显抑制作用。囊性纤维化跨膜电导调节因子也被干扰素-γ下调，这在蛋白质水平和cAMP依赖电流的减少上是明显的。另一方面，干扰素-γ可通过激活钙依赖的氯离子通道而使UTP诱导的电流增加。肿瘤坏死因子-α对离子转运几乎没有影响。在正常和囊性纤维化细胞中测量跨上皮液转运。在静息状态下，两种类型的细胞都表现出对阿米洛利敏感的液体吸收，这种吸收被干扰素-γ抑制，但不被肿瘤坏死因子-α抑制。我们的结果表明，干扰素-γ改变了培养的支气管细胞的跨上皮离子转运。这种效应可能会改变睫状液的离子组成和/或体积。
Human bronchial epithelial cells were treated in vitro with interferon-gamma or tumor necrosis factor-alpha to assess their effect on transepithelial ion transport. Short-circuit current measurements revealed that Na+ absorption was markedly inhibited by interferon-gamma (10-1,000 U/ml). The cystic fibrosis transmembrane conductance regulator was also downregulated by interferon-gamma as evident at the protein level and by the decrease in the cAMP-dependent current. On the other hand, interferon-gamma caused an increase of the current elicited by apical UTP application, which is due to the activity of Ca2+-dependent Cl- channels. Tumor necrosis factor-alpha caused few changes in ion transport. Transepithelial fluid transport was measured in normal and cystic fibrosis cells. At rest, both types of cells showed an amiloride-sensitive fluid absorption that was inhibited by interferon-gamma but not by tumor necrosis factor-alpha. Our results show that interferon-gamma alters the transepithelial ion transport of cultured bronchial cells. This effect may change the ion composition and/or volume of periciliary fluid.