The anti-mutagenic and antioxidant effects of bile pigments in the Ames Salmonella test
The anti-mutagenic and antioxidant effects of bile pigments in the Ames Salmonella test
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DOI：
10.1016/j.mrgentox.2007.01.008
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发表时间：
2007-05-18
影响因子：
1.9
通讯作者：
Wagner, K-H
中科院分区：
文献类型：
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作者：
Bulmer, A. C.;Ried, K.;Wagner, K-H
本研究旨在探讨内源性胆色素未结合胆红素（BR）、胆绿素（BV）和合成的水溶性结合物胆红素二月桂酸盐（BRT）在艾姆斯沙门氏菌试验中的潜在促突变和抗突变作用。在存在三种细菌菌株（TA 98、TA 100、TA 102）的情况下，在宽浓度范围（0.01-2 μ mol/板）内检测胆色素。采用苯并[α]芘（B[α]P）、2，4，7-三硝基芴酮（TNF one）、2-氨基芴（2-AF）、叠氮化钠（NaN 3）和叔丁基过氧化氢（t-BuOOH）等诱变剂促进突变体的形成。在存在（B[α]P，2-AF，t-BuOOH）和不存在（TNF酮，NaN 3，t-BuOOH）代谢活化的情况下进行试验，并加入微粒体肝脏制备物S9。单独的胆色素在任何试验菌株中均未诱导致突变性（p > 0.05）。在除NaN 3外的所有诱变剂存在下观察到胆色素的抗诱变作用，并且抗诱变作用似乎与供试菌株无关。对于TNF-α诱导的遗传毒性，有效性的顺序为BR >= BRT > BV。TA 102菌株的抗氧化试验表明，胆色素能有效地抑制t-BuOOH诱导的氧化应激的遗传毒性作用。胆色素的明显抗氧化和抗突变行为进一步表明它们在生物系统中的存在可能具有生理重要性。(c)2007 Elsevier B. V.保留所有权利。
The aim of this study was to explore the potential pro- and anti-mutagenic effects of endogenous bile pigments unconjugated bilirubin (BR), biliverdin (BV) and a synthetic, water soluble conjugate, bilirubin ditaurate (BRT) in the Ames Salmonella test. The bile pigments were tested over a wide concentration range (0.01-2 mu mol/plate) in the presence of three bacterial strains (TA98, TA100, TA102). A variety of mutagens including benzo[alpha]pyrene (B[alpha]P), 2,4,7 trinitrofluorenone (TNFone), 2-aminotluorene (2-AF), sodium azide (NaN3) and tertiary-butyl hydroperoxide (t-BuOOH), were used to promote the formation of mutant revertants. Tests were conducted with (B[alpha]P, 2-AF, t-BuOOH) and without (TNFone, NaN3, t-BuOOH) metabolic activation incorporating the addition of the microsomal liver preparation, S9. The bile pigments alone did not induce mutagenicity in any of the strains tested (p > 0.05). Anti-mutagenic effects of the bile pigments were observed in the presence of all mutagens except for NaN3 and the anti-mutagenic effects appeared independent of the strain tested. For TNFone induced genotoxicity, the order of effectiveness was BR >= BRT > BV. However, the order was BV >= BRT >= BR for 2-AF. Antioxidant testing in the TA102 strain revealed bile pigments could effectively inhibit the genotoxic effect of t-BuOOH induced oxidative stress. The apparent antioxidant and anti-mutagenic behaviour of bile pigments further suggests their presence in biological systems is of possible physiological importance. (c) 2007 Elsevier B.V. All rights reserved.