Physical Location of New Stripe Rust Resistance Gene(s) and PCR-Based Markers on Rye (Secale cereale L.) Chromosome 5 Using 5R Dissection Lines
Physical Location of New Stripe Rust Resistance Gene(s) and PCR-Based Markers on Rye (Secale cereale L.) Chromosome 5 Using 5R Dissection Lines
复制标题
使用 5R 解剖线对黑麦 (Secalealee L.) 5 号染色体上新的条锈病抗性基因和基于 PCR 的标记进行物理定位
DOI：
10.3390/agronomy9090498
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发表时间：
2019-09-01
期刊：
影响因子：
3.7
通讯作者：
Fu, Shulan
中科院分区：
文献类型：
--
作者：
Xi, Wei;Tang, Zongxiang;Fu, Shulan
黑麦（Secale cereale L.）5 R染色体上含有一些优良基因，可用于小麦品种的改良。本研究获得了一组5 R（Ku）分离系，并利用特异长度扩增片段测序（SLAF-seq）方法开发了111个新的基于PCR的5 R（Ku）特异性标记。将这111个标记与52个已报道的5 R（Ku）特异标记和65个S.使用5 R（Ku）解剖系将cereale Lo 7支架物理定位到5 R（Ku）染色体的六个区域。另外，5 RL（Ku）臂携带抗条锈基因，位于L2区，与22个5 R（Ku）特异标记和11个S. cereale Lo 7支架被定位。位于5 RL（Ku）臂上的抗条锈基因可能是新的抗条锈基因，其来源和位置与以往报道的不同，为小麦抗条锈育种提供了新的抗条锈基因来源。标记物和S.定位于5 R（Ku）染色体6个区域的禾谷类Lo 7支架有助于利用5 R染色体上的优良基因改良小麦品种。
The rye (Secale cereale L.) 5R chromosome contains some elite genes that can be used to improve wheat cultivars. In this study, a set of 5R(Ku) dissection lines was obtained, and 111 new PCR-based and 5R(Ku)-specific markers were developed using the specific length amplified fragment sequencing (SLAF-seq) method. The 111 markers were combined with the 52 5R(Ku)-specific markers previously reported, and 65 S. cereale Lo7 scaffolds were physically mapped to six regions of the 5R(Ku) chromosome using the 5R(Ku) dissection lines. Additionally, the 5RL(Ku) arm carried stripe rust resistance gene(s) and it was located to the region L2, the same region where 22 5R(Ku)-specific markers and 11 S. cereale Lo7 scaffolds were mapped. The stripe rust resistance gene(s) located in the 5RL(Ku) arm might be new one(s) because its source and location are different from the previously reported ones, and it enriches the resistance source of stripe rust for wheat breeding programs. The markers and the S. cereale Lo7 scaffolds that were mapped to the six regions of the 5R(Ku) chromosome can facilitate the utilization of elite genes on the 5R chromosome in the improvement of wheat cultivars.