Magnetite nanoparticles coated with mercaptosuccinic acid-modified mesoporous titania as a hydrophilic sorbent for glycopeptides and phosphopeptides prior to their quantitation by LC-MS/MS
Magnetite nanoparticles coated with mercaptosuccinic acid-modified mesoporous titania as a hydrophilic sorbent for glycopeptides and phosphopeptides prior to their quantitation by LC-MS/MS
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DOI：
10.1007/s00604-019-3274-3
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发表时间：
2019-03-01
影响因子：
5.7
通讯作者：
Deng, Chunhui
中科院分区：
文献类型：
--
作者：
Sun, Nianrong;Wang, Jiawen;Deng, Chunhui
制备了一种由巯基丁二酸改性的介孔二氧化钛包覆的磁铁矿核组成的亲水性材料（表示为Fe3O4@mTiO2-MSA）。它被证明是一个可行的吸附剂捕获糖肽和磷酸肽。该吸附剂结合了基于金属氧化物的亲和色谱和亲水相互作用液相色谱（HILIC）的特点，并具有使用介孔二氧化钛的优点。磁性微球的使用提供了磁性响应并简化了分离。用10%氨洗脱后，将肽进行LC-MS/ MS分析。该方法使327磷酸肽和65糖肽被确定在三个单独的重复，只有5 μ L的人唾液样品。其中，在20个肽段中检测到磷酸化位点和糖基化位点。
A hydrophilic material consisting of a magnetite core coated with mercaptosuccinic acid modified mesoporous titania (denoted as Fe3O4@ mTiO2-MSA) has been fabricated. It is shown to be a viable sorbent for capturing glycopeptides and phosphopeptides. The sorbent combines the features of metal oxide-based affinity chromatography and of hydrophilic interaction liquid chromatography (HILIC) with the advantages of using mesoporous titania. The use of magnetic microspheres provides magnetic response and simplifies separation. Following elution with 10% ammonia, the peptides were submitted to LC-MS/ MS analysis. The method enabled 327 phosphopeptides and 65 glycopeptides to be identified in three isolated replicates of merely 5 mu L samples of human saliva. Among them, the phosphorylation sites and glycosylation sites were detected in 20 peptide segments.