Preferential lectin binding of cancer cells upon sialic acid treatment under nutrient deprivation.
Preferential lectin binding of cancer cells upon sialic acid treatment under nutrient deprivation.
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DOI：
10.1007/s12010-013-0409-6
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发表时间：
2013-10
影响因子：
3
通讯作者：
Djansugurova LB
中科院分区：
文献类型：
--
作者：
Badr HA;Elsayed AI;Ahmed H;Dwek MV;Li CZ;Djansugurova LB
真核细胞表面糖缀合物的末端单糖通常是唾液酸（Neu5Ac）。唾液化升高通常预示着大多数癌的进展和预后不良。在这里，我们利用两种人类乳腺上皮细胞系HB4A（乳腺正常细胞）和T47D（乳腺癌细胞）作为模型系统，证明在营养剥夺的情况下，唾液酸处理后的表面聚糖的差异。凝集素结合固相测定表明，在饥饿条件下，唾液酸处理的两种细胞α2→3/6唾液基转移酶活性均增加。然而，唾液酸处理后的T47D细胞膜上有很强的MAL-I （Maackia amurensis凝集素I）染色，表明细胞表面Neu5Acα2→3Gal增加。据我们所知，这是第一个显示凝集素，特别是mal - 1，在营养剥夺下唾液酸处理后作为区分正常细胞和癌细胞的手段的报告。这种方法是敏感的，可以选择性地检测癌细胞表面的多糖唾液化。
The terminal monosaccharide of glycoconjugates on a eukaryotic cell surface is typically a sialic acid (Neu5Ac). Increased sialylation usually indicates progression and poor prognosis of most carcinomas. Here, we utilize two human mammary epithelial cell lines, HB4A (breast normal cells) and T47D (breast cancer cells) as a model system to demonstrate differential surface glycans when treated with sialic acid under nutrient deprivation. Under a starved condition, sialic acid treatment of both cells resulted in increased activities of α2→3/6 sialyltransferases as demonstrated by solid phase assay using lectin binding. However, a very strong MAL-I (Maackia amurensis agglutinin I) staining on the membrane of sialic acid treated T47D cells was observed, indicating an increase of Neu5Acα2→3Gal on the cell surface. To our knowledge, this is a first report showing the utility of lectins, particularly MAL-I, as a means to discriminate between normal and cancer cells after sialic acid treatment under nutrient deprivation. This method is sensitive and allows selectively detection of glycan sialylation on a cancer cell surface.
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影响因子：
64.5
作者：
Lau, Ken S.;Partridge, Emily A.;Dennis, James W.
通讯作者：
Dennis, James W.
影响因子：
15
作者：
Schwefel, David;Maierhofer, Caroline;Wittmann, Valentin
通讯作者：
Wittmann, Valentin
影响因子：
8.2
作者：
Qian, Jin;Zhu, Cai-hua;Ding, Jian
通讯作者：
Ding, Jian
影响因子：
3.8
作者：
Meany DL;Chan DW
通讯作者：
Chan DW
DOI：
10.1039/c2cc34853e
发表时间：
2012-10-28
期刊：
Chemical communications (Cambridge, England)
影响因子：
--
作者：
Liu C;Lei T;Ino K;Matsue T;Tao N;Li CZ
通讯作者：
Li CZ