puckered encodes a phosphatase that mediates a feedback loop regulating JNK activity during dorsal closure in Drosophila
puckered encodes a phosphatase that mediates a feedback loop regulating JNK activity during dorsal closure in Drosophila
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DOI：
10.1101/gad.12.4.557
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发表时间：
1998-02-15
影响因子：
10.5
通讯作者：
Martinez-Arias, A
中科院分区：
文献类型：
--
作者：
Martín-Blanco, E;Gampel, A;Martinez-Arias, A
MAPK 的激活由 MAPK 激酶和 MAPK 磷酸酶活性之间的平衡控制。后者由具有双重特异性的磷酸酶子集（VH-1 家族）介导。在这里，我们描述了这个家族的一个新成员，由果蝇的褶皱基因编码。该基因的突变会导致细胞骨架缺陷，从而导致与篮子（果蝇 INK 同源物）突变相关的背侧闭合失败。我们发现褶皱突变会导致 DJNK 过度激活，并且 puc 的过度表达模仿篮子突变体表型。我们还表明，puckered 表达本身就是 JNK 通路活性的结果，并且在背侧闭合过程中，JNK 信号传导具有双重作用：激活由 decapentaplegic 编码的效应器，以及由 puckered 编码的负反馈调节元件。
The activation of MAPKs is controlled by the balance between MAPK kinase and MAPK phosphatase activities. The latter is mediated by a subset of phosphatases with dual specificity (VH-1 family). Here, we describe a new member of this family encoded by the puckered gene of Drosophila. Mutations in this gene lead to cytoskeletal defects that result in a failure in dorsal closure related to those associated with mutations in basket, the Drosophila INK homolog. We show that puckered mutations result in the hyperactivation of DJNK, and that overexpression of puc mimics basket mutant phenotypes. We also show that puckered expression is itself a consequence of the activity of the JNK pathway and that during dorsal closure, JNK signaling has a dual role: to activate an effector, encoded by decapentaplegic, and an element of negative feedback regulation encoded by puckered.