Label-free detection of low protein concentration in solution using a novel colorimetric assay
Label-free detection of low protein concentration in solution using a novel colorimetric assay
复制标题
DOI：
10.1016/j.bios.2013.05.011
复制
发表时间：
2013-11-15
影响因子：
12.6
通讯作者：
Serpe, Michael J.
中科院分区：
文献类型：
--
作者：
Islam, Molla R.;Serpe, Michael J.
通过将“单片”微凝胶层夹在两个半透明的Au层之间，制备了双pH和温度敏感的微凝胶基标准子。该器件表现出视觉颜色和多峰反射光谱，这两者主要取决于由微凝胶直径介导的Au表面之间的距离。我们发现，一种聚阳离子聚电解质可以穿透金层，与被限制在金层之间的带负电的微凝胶相互作用。在这篇论文中，我们报道了生物素化的聚阳离子聚合物可以穿透聚n -异丙基丙烯酰胺-共丙烯酸（pNIPAm-co-AAc）微凝胶基标准子的Au层并导致微凝胶层坍塌。塌缩导致反射光谱的光谱峰发生移位。我们发现，峰移的程度取决于加入到标准子上的生物素化多阳离子的量，这可以随后用于确定pM浓度下溶液中链霉亲和素的浓度。(C) 2013 Elsevier B.V.版权所有
Dual pH and temperature sensitive microgel-based etalons were fabricated by sandwiching a "monolithic" microgel layer between two semitransparent, Au layers. The devices exhibit visual color and multipeak reflectance spectra, both of which primarily depend on the distance between the Au surfaces mediated by the microgel diameter. We found that a polycationic polyelectrolyte can penetrate through the Au overlayer to interact with negatively charged microgel confined between Au overlayers. In this submission we report that biotinylated polycationic polymer can penetrate through the Au overlayer of a poly (N-isopropylacrylamide)-co-acrylic acid (pNIPAm-co-AAc) microgel-based etalon and cause the microgel layer to collapse. The collapse results in a shift in the spectral peaks of the reflectance spectra. We found that the extent of peak shift depends on the amount of biotinylated polycation added to the etalon, which can subsequently be used to determine the concentration of streptavidin in solution at pM concentrations. (C) 2013 Elsevier B.V. All rights reserved.