Extracellular Matrix Membrane Induces Cementoblastic/Osteogenic Properties of Human Periodontal Ligament Stem Cells
Extracellular Matrix Membrane Induces Cementoblastic/Osteogenic Properties of Human Periodontal Ligament Stem Cells
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DOI：
10.3389/fphys.2018.00942
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发表时间：
2018-07
影响因子：
4
通讯作者：
Yuanyuan Wang;S. Papagerakis;D. Faulk;S. Badylak;Yuming Zhao;L. Ge;M. Qin;P. Papagerakis
中科院分区：
文献类型：
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作者：
Yuanyuan Wang;S. Papagerakis;D. Faulk;S. Badylak;Yuming Zhao;L. Ge;M. Qin;P. Papagerakis
目的：牙周炎影响近 90% 的 70 岁以上成年人，导致牙周组织感染、破坏，最终导致牙齿脱落。引导组织再生（GTR）是一种广泛用于治疗严重牙周病的方法，涉及放置闭塞屏障以促进牙周膜干细胞（PDLSC）对受损区域进行再生。在本研究中，我们评估天然细胞外基质（ECM）作为支架材料，以提供合适的微环境来支持 PDLSC 的增殖、分化和组织再生特性。设计：将在从猪膀胱中分离的 ECM 膜上培养的 PDLSC 的活力、增殖、凋亡和迁移与在 I 型胶原膜（GTR 中常用的支架）上培养的 PDLSC 进行比较。为了评估 ECM 与 I 型胶原对 PDLSC 组织再生特性的影响，评估了 PDLSC 的生物附着和成牙骨质细胞/成骨分化。结果：与 I 型胶原处理的 PDLSC 相比，用 ECM 孵育 PDLSC 可提高活力、增殖并减少细胞凋亡。与 ECM 膜共培养还增加了 PDLSC 的迁移和生物附着。 PDLSC 与 ECM 膜一起孵育可增加成牙骨质细胞/成骨分化标志物 BSP、RUNX2、ALP、OPN、OCN 和骨膜素的表达。结论：ECM膜增强PDLSC的增殖和再生特性，表明ECM膜可以作为GTR治疗牙周病的合适支架。
Objective: Periodontitis affects nearly 90% of adults over the age of 70, resulting to periodontal tissue infection, destruction, and ultimately tooth loss. Guided tissue regeneration (GTR) is a method widely used to treat severe periodontal disease, and involves placement of an occlusive barrier to facilitate regeneration of the damaged area by periodontal ligament stem cells (PDLSCs). In this study, we evaluate natural extracellular matrix (ECM) as a scaffold material to provide a suitable microenvironment to support the proliferation, differentiation, and tissue-regenerating properties of PDLSCs. Design: The viability, proliferation, apoptosis, and migration of PDLSCs cultured on ECM membrane, that was isolated from porcine urinary bladders, were compared with those cultured on type I collagen membrane, a commonly used scaffold in GTR. To evaluate the effects of ECM vs. type I collagen on the tissue-regenerating properties of PDLSCs, the bio-attachment and cementoblastic/osteogenic differentiation of PDLSCs were evaluated. Results: Incubation of PDLSCs with ECM resulted in increased viability, proliferation, and reduced apoptosis, compared with type I collagen treated PDLSCs. Co-culture with ECM membrane also increased the migration and bio-attachment of PDLSCs. Incubation of PDLSCs with ECM membrane increased expression of the cementoblastic/osteogenic differentiation markers BSP, RUNX2, ALP, OPN, OCN, and periostin. Conclusion: ECM membrane enhances the proliferation and regenerative properties of PDLSCs, indicating that ECM membrane can serve as a suitable scaffold in the application of GTR to treat periodontal disease.