Specific ammonium ion requirement for functional ribosomal RNA tertiary structure.
Specific ammonium ion requirement for functional ribosomal RNA tertiary structure.
复制标题
功能性核糖体 RNA 三级结构所需的特定铵离子。
DOI：
10.1021/bi00097a002
复制
发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Draper,DE
中科院分区：
文献类型：
--
作者：
Wang,YX;Lu,M;Draper,DE
MATERIALS AND METHODSRNA Preparation and Melting Experiments. The Es-cherichia coli large-subunit ribosomal RNA sequence G1051-U1158 was synthesized from plasmid DNA by T7 RNA polymerase runoff transcription as described (Ryan & Draper, 1989; Laing & Draper, 1993), using a clone which could be cleaved with Rsal to give the 3'terminus shown in Figure 1. The RNA was purified by column gel electrophoresis, using 8 M urea and 8% acrylamide. Melting data were collected in a Perkin-Elmer Lambda 4 spectrophotometer interfaced to a computer and temperature probe (Laing & Draper, 1993). t This work was supported by NIH Grants GM37005 and GM29048.* To whom correspondence shouldbe addressed.* Abstract published in Advance ACS Abstracts, November 1, 1993.
