The plant-specific transcription factors CBP60g and SARD1 are targeted by a Verticillium secretory protein VdSCP41 to modulate immunity.
The plant-specific transcription factors CBP60g and SARD1 are targeted by a Verticillium secretory protein VdSCP41 to modulate immunity.
复制标题
植物特异性转录因子 CBP60g 和 SARD1 是黄萎病分泌蛋白 VdSCP41 的靶标，可调节免疫力
DOI：
10.7554/elife.34902
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发表时间：
2018-05-14
期刊：
影响因子：
7.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Qin J;Wang K;Sun L;Xing H;Wang S;Li L;Chen S;Guo HS;Zhang J
维管束病原菌黄萎病侵染植物根部，引起黄萎病。大丽弧菌毒力和寄主抗性的分子机制仍不清楚。在这里，我们证明了一种分泌蛋白VdSCP41作为细胞内效应器来促进大丽弧菌的毒力。拟南芥主免疫调节剂CBP60g和SARD1和棉花GhCBP60b是VdSCP41的靶标。VdSCP41结合CBP60g的C-末端部分以抑制其转录因子活性。进一步的分析表明，CBP60g中有一个转录激活结构域，是VdSCP41靶向所必需的。CBP60g和SARD1的突变降低了拟南芥对大丽弧菌的抗性，并部分削弱了VdSCP41介导的毒力。此外，病毒诱导的GhCBP60b沉默降低了棉花对大丽叶病毒的抗性。这项工作揭示了大丽弧菌分泌蛋白VdSCP41直接针对植物转录因子抑制免疫的毒力策略，并揭示了CBP60g、SARD1和GhCBP60b是控制大丽弧菌抗性的关键成分。
The vascular pathogen Verticillium dahliae infects the roots of plants to cause Verticillium wilt. The molecular mechanisms underlying V. dahliae virulence and host resistance remain elusive. Here, we demonstrate that a secretory protein, VdSCP41, functions as an intracellular effector that promotes V. dahliae virulence. The Arabidopsis master immune regulators CBP60g and SARD1 and cotton GhCBP60b are targeted by VdSCP41. VdSCP41 binds the C-terminal portion of CBP60g to inhibit its transcription factor activity. Further analyses reveal a transcription activation domain within CBP60g that is required for VdSCP41 targeting. Mutations in both CBP60g and SARD1 compromise Arabidopsis resistance against V. dahliae and partially impair VdSCP41-mediated virulence. Moreover, virus-induced silencing of GhCBP60b compromises cotton resistance to V. dahliae. This work uncovers a virulence strategy in which the V. dahliae secretory protein VdSCP41 directly targets plant transcription factors to inhibit immunity, and reveals CBP60g, SARD1 and GhCBP60b as crucial components governing V. dahliae resistance.