Identification and Characterization of Secreted Proteins from Magnaporthe Grisea
Identification and Characterization of Secreted Proteins from Magnaporthe Grisea
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DOI：
10.1007/978-0-306-48582-4_6
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
G. Lu;C. Filippi;Dan Li;D. Ebbole
中科院分区：
文献类型：
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作者：
G. Lu;C. Filippi;Dan Li;D. Ebbole
稻瘟病（Magnaporthe grisea）每年通过直接作物损失或间接杀菌剂成本造成重大经济损失。产生有效耐药性的一个关键是更好地了解宿主-病原体相互作用，这需要对宿主和病原体都有透彻的了解。通过主要抗病基因识别稻瘟病真菌通常涉及寄主植物对真菌特异性分子的检测。这些识别分子主要被认为是真菌分泌的蛋白质。迄今为止研究的分泌蛋白是植物细胞壁降解酶，如两种木聚糖酶。griseaXYN22和XYN33 （Wu et ., 1995）和细粒曲霉的聚半乳糖醛酸酶和果胶裂解酶（Dean and Timberlake, 1989）；与毒力有关的蛋白质，如黄枝孢子菌的ECP1和ECP2， m的gas1和GAS2。小麦分别是番茄和水稻的毒力因子（Lauge et al., 1997; Xue et al., 2002）；MPG1胶卷暗盒。小麦参与致病性（Talbot et al., 1993）；或无毒基因产物，如m的Avr-Pita和Pwl2基因。grisea(Orbach et al.， 2000和Sweigard et al., 1995)；大麦烫伤病原菌稻瘟霉（rynchosporium secalis, Rohe et, 1995）的NIP 1和c的AVR4、AVR9、ECP1、ECP2、ECP3、ECP4和ECP5。fulvum（Joosten and de Wit, 1999; Lauge et al., 2000）。对稻瘟病菌分泌的蛋白质进行更系统的检查将有助于确定与植物寄主相互作用的真菌因子。
Rice blast disease (Magnaporthe grisea) causes significant economic damage every year either directly through crop loss or indirectly in the cost of fungicides. A key to developing effective resistance is gaining a better understanding of the host-pathogen interaction, which requires a thorough understanding of both the host and pathogen. Recognition of the rice blast fungus via major disease resistance genes generally involves detection of a fungal-specific molecule by the host plant. These recognition molecules are primarily thought to be proteins secreted by the fungus. Secreted proteins examined to date have been plant cell wall-degrading enzymes, such as two xylanases ofM. griseaXYN22 and XYN33 (Wu et al., 1995) and polygalacturonase and pectate lyase ofAspergillus nidulans(Dean and Timberlake, 1989); proteins involved in virulence, such as ECP1 and ECP2 ofCladosporium fulvum, and GAS 1 and GAS2 ofM. griseaare the virulence factors to tomato and rice, respectively (Lauge et al., 1997; Xue et al., 2002); MPG1 ofM. griseainvolved in pathogenecity (Talbot et al., 1993); or avirulence gene products, such as Avr-Pita and Pwl2 gene ofM. grisea(Orbach et al., 2000 and Sweigard et al., 1995); NIP 1 of barley scald pathogenRynchosporium secalis(Rohe et al., 1995) and AVR4, AVR9, ECP1, ECP2, ECP3, ECP4 and ECP5 ofC. fulvum(Joosten and de Wit, 1999 and Lauge et al., 2000). A more systematic examination of secreted proteins from the rice blast fungus would help to identify the fungal factors involved in the interaction with the plant host.