Seeding Assay Using Cultured Cells
Seeding Assay Using Cultured Cells
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使用培养细胞进行接种测定
DOI：
10.1007/978-1-0716-1495-2_3
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Hattori Nobutaka
中科院分区：
文献类型：
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作者：
Ogata Jun;Takemoto Daisaku;Shimonaka Shotaro;Imai Yuzuru;Hattori Nobutaka
α-突触核蛋白是一种突触前蛋白，参与响应神经元活动的突触小泡动力学。 α-突触核基因的突变和 α-突触核蛋白的神经元沉积（称为路易体）与帕金森病的发展有关。 α-突触核蛋白具有类似朊病毒的特性，可将其生理蛋白质构象转化为致病性蛋白质构象，形成致病原纤维。这些原纤维的聚集和随后的包涵体形成被认为会干扰神经元中的囊泡运输和细胞器功能。因此，需要检测 α-突触核蛋白的朊病毒样特性并评估其修饰因子，以了解帕金森病的发病机制并开发新疗法。在本章中，我们描述了一种基于细胞的检测 α-突触核蛋白增殖的方法。
α-Synuclein, a presynaptic protein, is involved in synaptic vesicle dynamics in response to neuronal activity. Mutations of theα-synucleingene and the neuronal deposition of α-synuclein, called Lewy bodies, are linked to the development of Parkinson’s disease. α-Synuclein has a prion-like property that converts its physiological protein conformation to a pathogenic one, forming disease-causing fibrils. Aggregation of these fibrils and subsequent inclusion formation are suggested to interfere with vesicular trafficking and organelle function in neurons. Thus, detection of a prion-like property of α-synuclein and the evaluation of its modifying factors are required to understand the pathogenesis of Parkinson’s disease and to develop new therapies. In this chapter, we describe a cell-based assay for detecting α-synuclein propagation.