ISOLATION OF THE BETA-TUBULIN GENE FROM YEAST AND DEMONSTRATION OF ITS ESSENTIAL FUNCTION INVIVO
ISOLATION OF THE BETA-TUBULIN GENE FROM YEAST AND DEMONSTRATION OF ITS ESSENTIAL FUNCTION INVIVO
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DOI：
10.1016/0092-8674(83)90350-1
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
BOTSTEIN, D
中科院分区：
文献类型：
--
作者：
NEFF, NF;THOMAS, JH;BOTSTEIN, D
从酵母中提取的DNA片段（S.酿酒酵母）通过其与鸡β-微管蛋白cDNA [互补DNA]并克隆。该片段在酵母基因组中是独特的，并含有酵母β-的基因。微管蛋白，因为它可以补充苯菌灵抗性条件致死突变。较小的亚片段，当用于指导质粒整合到二倍体细胞中的苯菌灵抗性基因座时，破坏了β-谷氨酰胺的1个。微管蛋白基因，并伴随产生隐性致死突变，表明单个β-微管蛋白基因的突变可能是一个突变。酵母微管蛋白基因具有重要的功能。核苷酸序列的测定揭示了酵母和鸡脑β之间广泛的氨基酸序列同源性（超过70%）。微管蛋白
A DNA fragment from yeast (S. cerevisiae) was identified by its homology to a chicken .beta.-tubulin cDNA [complementary DNA] and cloned. The fragment was unique in the yeast genome and to contain the gene for yeast .beta.-tubulin, since it can complement a benomyl-resistant conditional-lethal mutation. A smaller subfragment, when used to direct integration of a plasmid to the benomyl resistance locus in a diploid cell, disrupted 1 of the .beta.-tubulin genes and concomitantly created a recessive lethal mutation, indicating that the single .beta.-tubulin gene of yeast has an essential function. Determination of the nucleotide sequence reveals extensive amino acid sequence homology (more than 70%) between yeast and chicken brain .beta.-tubulins.