RELATIVE DENSITIES OF MUSCARINIC CHOLINERGIC AND BETA-ADRENERGIC RECEPTORS IN THE CANINE SINOATRIAL NODE AND THEIR RELATION TO SITES OF PACEMAKER ACTIVITY
RELATIVE DENSITIES OF MUSCARINIC CHOLINERGIC AND BETA-ADRENERGIC RECEPTORS IN THE CANINE SINOATRIAL NODE AND THEIR RELATION TO SITES OF PACEMAKER ACTIVITY
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DOI：
10.1161/01.res.77.5.957
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发表时间：
1995-11-01
影响因子：
20.1
通讯作者：
SAFFITZ, JE
中科院分区：
文献类型：
--
作者：
BEAU, SL;HAND, DE;SAFFITZ, JE
已知窦房结最早细胞外电活动的部位会因自主神经刺激而移位，但这种现象背后的机制和主要起搏器活动位置的决定因素尚未阐明。本研究旨在研究犬窦房结内M胆碱能和β-肾上腺素能受体的空间分布，并确定自主神经受体密度与优势起搏器活动部位之间是否存在一致的关系。我们用放射配基结合部位的定量光镜放射自显影技术，研究了18只犬心脏窦房结和邻近右心房肌组织切片中M胆碱能和β-肾上腺素能受体亚型的空间分布。窦房结细胞的M受体密度是心房肌细胞的5.4倍(P
The site of earliest extracellular electrical activation in the sinoatrial node (SAN) is known to shift in response to autonomic stimuli, but the mechanisms underlying this phenomenon and the determinants of the location of dominant pacemaker activity have not been elucidated. The present study was designed to characterize the spatial distribution of muscarinic cholinergic and beta-adrenergic receptors in the canine SAN and to determine whether a consistent relationship exists between autonomic receptor densities and the site of dominant pacemaker activity. We used quantitative light-microscopic autoradiography of radioligand binding sites to characterize the spatial distribution of muscarinic cholinergic and beta-adrenergic receptor subtypes in tissue sections containing the SAN and adjacent right atrial muscle from 18 canine hearts. Muscarinic receptor density was 5.4 times greater in SAN cells than in atrial myocytes (P