REGULATION OF INSULIN-LIKE GROWTH FACTOR-I GENE-EXPRESSION BY GROWTH-HORMONE
REGULATION OF INSULIN-LIKE GROWTH FACTOR-I GENE-EXPRESSION BY GROWTH-HORMONE
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DOI：
10.1073/pnas.83.24.9343
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
PALMITER, RD
中科院分区：
文献类型：
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作者：
MATHEWS, LS;NORSTEDT, G;PALMITER, RD
从小鼠基因组文库中分离出含有胰岛素样生长因子 I (IGF-I) 基因外显子 3 的重组克隆。这些序列用于生成 RNA 探针，该探针用于溶液杂交测定中，以根据生长激素状态来定量各种鼠组织中的 IGF-I mRNA。肝脏是 IGF-I 合成的主要部位，在生长激素缺乏的 lit/lit 小鼠中，IGF-I mRNA 的水平受生长激素调节约 10 倍。核连载测定用于表明生长激素调节部分表现在转录水平。生长激素还影响肝脏 IGF-I mRNA 的大小分布。胰腺显示出最高的非肝脏表达，但分析的每个组织都含有一些 IGF-I mRNA。在大多数非肝组织中表达不依赖于生长激素。
Recombinant clones containing exon 3 of the insulin-like growth factor I (IGF-I) gene were isolated from a mouse genomic library. These sequences were used to generate an RNA probe, which was used in a solution hybridization assay to quantitate IGF-I mRNA in various murine tissues as a function of growth hormone status. The liver is the major site of IGF-I synthesis and the level of IGF-I mRNA is regulated about 10-fold by growth hormone in the growth hormone-deficient lit/lit mouse. Nuclear run-on assays were used to show that growth hormone regulation is manifested in part at the transcriptional level. Growth hormone also affects the size distribution of hepatic IGF-I mRNAs. Pancreas showed the highest non-hepatic expression, but every tissue analyzed contained some IGF-I mRNA. Expression was not growth hormone-dependent in most non-hepatic tissues.