Nerve injury enhances rat neuronal glutamate transporter expression: identification by differential display PCR
Nerve injury enhances rat neuronal glutamate transporter expression: identification by differential display PCR
复制标题
神经损伤增强大鼠神经元谷氨酸转运蛋白表达：通过差异显示 PCR 进行鉴定
DOI：
10.1523/jneurosci.15-12-07872.1995
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发表时间：
1995
影响因子：
3.5
通讯作者：
H. Kiyama
中科院分区：
文献类型：
--
作者：
S. Kiryu;G. Yao;N. Morita;H. Kato;H. Kiyama
通过差异显示PCR（DD-PCR）结合原位杂交技术，证实了神经损伤后神经元谷氨酸转运体表达的增加。采用DD-PCR方法比较切断轴突和正常舌下神经运动神经元mRNA表达的差异。几个基因片段的表达被发现增加神经损伤后，相应的一个片段的全长cDNA克隆通过随后的大鼠cDNA文库筛选。谷氨酸转运蛋白与我们的大鼠cDNA的密切同源性使我们得出结论，该克隆对应于大鼠神经元谷氨酸转运蛋白（大鼠EAAC 1）。我们推测，这种谷氨酸摄取系统的上调可能会增加这些细胞对神经毒性谷氨酸积累的神经再生过程中的阻力。
An increase in neuronal glutamate transporter expression after nerve injury was demonstrated by means of differential display PCR (DD-PCR) coupled with in situ hybridization. DD-PCR was carried out to compare differences in expression of mRNAs between axotomized and normal hypoglossal motoneurons in the rat. The expression of several gene fragments were found to be increased following nerve injury; the full length cDNA corresponding to one fragment was cloned by subsequent rat cDNA library screening. The close homology of glutamate transporters with our rat cDNA led us to conclude that this clone corresponds to the rat neuronal glutamate transporter (rat EAAC1). We speculate that the upregulation of this glutamate uptake system may increase the resistance of these cells against neurotoxic glutamate accumulation during the process of nerve regeneration.
影响因子：
158.5
作者：
ROTHSTEIN, JD;MARTIN, LJ;KUNCL, RW
通讯作者：
KUNCL, RW
DOI：
10.1073/pnas.84.23.8735
发表时间：
1987-12-01
影响因子：
11.1
作者：
HEUMANN, R;LINDHOLM, D;THOENEN, H
通讯作者：
THOENEN, H