Synthesis and characterization of anti-EGFR fluorescent nanoparticles for optical molecular imaging.
Synthesis and characterization of anti-EGFR fluorescent nanoparticles for optical molecular imaging.
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DOI：
10.1021/bc300355y
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发表时间：
2013-02-20
影响因子：
4.7
通讯作者：
Pun, Suzie H.
中科院分区：
文献类型：
--
作者：
Chan, Leslie W.;Wang, Yak-Nam;Lin, Lih Y.;Upton, Melissa P.;Hwang, Joo Ha;Pun, Suzie H.
Molecular imaging, the visualization of molecular and cellular markers, is a promising method for detection of dysplasia and early cancer in the esophagus and can potentially be used to identify regions of interest for biopsy or tumor margins for resection. EGFR is a previously-reported cell surface receptor with stepwise increases in expression during the progression from Barrett’s metaplasia to adenocarcinoma. In this work, a 200-nm fluorescent nanoparticle contrast agent was synthesized for targeted imaging of EGFR through a series of surface modifications to dye-encapsulated polystyrene particles. Amino-functionalized polystyrene particles were PEGylated using a heterobifunctional PEG linker. Subsequently, thiolated M225 antibodies were conjugated to maleimide functional groups on attached PEGs for EGFR targeting. In vitro binding studies using flow cytometry demonstrated specific binding of M225-PEG-NP to EGFR-expressing cells with minimal non-specific binding in EGFR− cells. Binding was shown to increase proportionally with the number of conjugated M225 antibodies. Adsorbed formulations with unmodified M225 antibodies, M225 + PEG-NP, were synthesized using the same antibody feeds used in M225-PEG-NP synthesis to determine the contribution of adsorbed antibodies to EGFR targeting. Adsorbed antibodies were less efficient at mediated nanoparticle targeting to EGFR than conjugated antibodies. Finally, M225-PEG-NP demonstrated binding to EGFR-expressing regions in human esophageal tissue sections.
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影响因子：
29.4
作者：
Li M;Anastassiades CP;Joshi B;Komarck CM;Piraka C;Elmunzer BJ;Turgeon DK;Johnson TD;Appelman H;Beer DG;Wang TD
通讯作者：
Wang TD
DOI：
10.1021/la200659z
发表时间：
2011-06-21
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Ma LL;Tam JO;Willsey BW;Rigdon D;Ramesh R;Sokolov K;Johnston KP
通讯作者：
Johnston KP
影响因子：
9
作者：
Heitmiller, RF;Redmond, M;Hamilton, SR
通讯作者：
Hamilton, SR
影响因子：
11.5
作者：
Diagaradjane, Parmeswaran;Orenstein-Cardona, Jacobo M.;Krishnan, Sunil
通讯作者：
Krishnan, Sunil
影响因子：
3.9
作者：
Chen, SF;Liu, LY;Jiang, SY
通讯作者：
Jiang, SY