C6 glioma cell-conditioned medium induces neurite outgrowth and survival of rat chromaffin cells in vitro: comparison with the effects of nerve growth factor.
C6 glioma cell-conditioned medium induces neurite outgrowth and survival of rat chromaffin cells in vitro: comparison with the effects of nerve growth factor.
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C6 神经胶质瘤细胞条件培养基在体外诱导大鼠嗜铬细胞的神经突生长和存活：与神经生长因子的效果比较。
DOI：
10.1073/pnas.81.7.2242
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发表时间：
1984
影响因子：
11.1
通讯作者：
A. Wilson
中科院分区：
文献类型：
--
作者：
K. Unsicker;J. Vey;H. Hofmann;T. Müller;A. Wilson
研究了大鼠 C6 胶质瘤细胞 (C6-CM) 条件培养基对培养物中肾上腺髓质细胞的存活、神经突形成和儿茶酚胺含量的影响，并与神经生长因子 (NGF) 的影响进行比较。从 10 天大的大鼠中分离肾上腺髓质细胞，培养 8 天后测定存活细胞和神经突延伸细胞的比例。在 C6-CM 存在的情况下，几乎所有种子细胞都能存活，并且 50% 出现神经炎过程。相比之下，NGF 不支持高于对照水平 (30%) 的存活，并诱导大约三分之一的存活细胞形成神经突。 C6-CM 和 NGF 对神经突生长没有相加作用。 C6-CM 介导的纤维生长不受生理浓度的糖皮质激素的抑制，从而消除了 NGF 诱导的神经突形成。 C6-CM和NGF均增加了培养物的儿茶酚胺含量并降低了肾上腺素的相对含量。然而，考虑到与 NGF 相比，C6-CM 对细胞存活的显着影响，C6-CM 导致每个细胞的儿茶酚胺含量减少。我们得出的结论是，肾上腺髓质细胞与神经嵴感觉交感细胞谱系的其他成员一样，对神经胶质条件培养基有反应。这种反应在数量和质量上都与 NGF 介导的反应不同。
The effects of medium conditioned by rat C6 glioma cells (C6-CM) on the survival, neurite formation, and catecholamine content of adrenal medullary cells in culture were investigated and compared with the effects of nerve growth factor (NGF). Adrenal medullary cells were isolated from 10-day-old rats and the proportions of surviving and neurite-extending cells were determined after 8 days in culture. In the presence of C6-CM virtually all seeded cells survived and 50% developed neuritic processes. In contrast, NGF did not support survival above control levels (30%) and induced neurite formation from approximately one-third of the surviving cells. C6-CM and NGF had no additive effects on neurite outgrowth. C6-CM-mediated fiber outgrowth was not inhibited by physiological concentrations of glucocorticoids which abolished the NGF-induced neurite formation. Both C6-CM and NGF increased the catecholamine content of the cultures and reduced the relative content of epinephrine. However, in view of its substantial effect on cell survival as compared to NGF, C6-CM caused a reduction of the catecholamine content per cell. We conclude that adrenal medullary cells, like other members of the sensory-sympathetic cell lineage of the neural crest, respond to glial-conditioned medium. This response differs both quantitatively and qualitatively from that mediated by NGF.