Cyclophilin C-associated protein and cyclophilin C mRNA are upregulated in penumbral neurons and microglia after focal cerebral ischemia
Cyclophilin C-associated protein and cyclophilin C mRNA are upregulated in penumbral neurons and microglia after focal cerebral ischemia
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DOI：
10.1038/sj.jcbfm.9600029
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发表时间：
2005-03-01
影响因子：
6.3
通讯作者：
Saito, N
中科院分区：
文献类型：
--
作者：
Shimizu, T;Imai, H;Saito, N
免疫亲素配体，如环孢素A和FK506在实验性脑卒中模型中具有神经保护作用，尽管其确切机制尚不清楚。亲环蛋白C相关蛋白（Cyclophilin C-associated protein, CyCAP）是免疫亲环蛋白C的天然细胞配体，通过下调促炎反应对内毒素具有保护作用。应用Northern blotting和原位杂交技术研究了CyCAP和亲环蛋白C mRNA在大鼠大脑中动脉闭塞缺血模型中的表达。CyCAP和亲环蛋白C mrna在正常脑神经元中普遍存在。在MCA闭塞后7天内，梗死周围区的神经元表达增加。NeuN反免疫染色证实神经元分布。这两种mrna在7天时主要在缺血核心的小胶质细胞中表达，用小胶质细胞标记物ED1免疫染色证实。通过Northern blot分析，第7天CyCAP和亲环蛋白C的含量增加了8.5倍(P
Immunophillin ligands, such as cyclosporin A and FK506 have neuroprotective effects in experimental stroke models, although the precise mechanism is unclear. Cyclophilin C-associated protein (CyCAP) is a natural cellular ligand for the immunophilin, cyclophilin C, and has a protective effect against endotoxins by downmodulating the proinflammatory response. Expressions of CyCAP and cyclophilin C mRNA in a rat middle cerebral artery (MCA) occlusion ischemia model were investigated by Northern blotting and in situ hybridization. Both CyCAP and cyclophilin C mRNAs were ubiquitously distributed in the neurons of the normal brain. Expression increased in neurons of the periinfarct zone up to 7 days after MCA occlusion. The neuronal distribution was confirmed by counterimmunostaining of NeuN. Both mRNAs were predominantly expressed in microglia of the ischemic core at 7 days, confirmed by immunostaining with the microglial marker, ED1. The quantification of CyCAP and cyclophilin C mill at 7 days by Northern blot analysis showed the 8.5-fold increase (P