Genetic Characterization of HIV Type 1 Tat Exon 1 from a Southern Indian Clinical Cohort: Identification of Unique Epidemiological Signature Residues
Genetic Characterization of HIV Type 1 Tat Exon 1 from a Southern Indian Clinical Cohort: Identification of Unique Epidemiological Signature Residues
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DOI：
10.1089/aid.2011.0380
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发表时间：
2012-09-01
影响因子：
1.5
通讯作者：
Prasad, Vinayaka R.
中科院分区：
文献类型：
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作者：
Neogi, Ujjwal;Gupta, Soham;Prasad, Vinayaka R.
HIV-1的转录反式激活因子（Tat）在病毒感染和发病中起重要作用。我们检测了来自印度南部102名血清阳性受试者的tat基因外显子1的遗传特征。数据库衍生的印度（n = 105）和全球（n = 413） HIV-1C序列也用于Tat开放阅读框（ORF）中的病毒流行病学特征模式分析。我们确定HIV-1C是最主要的遗传亚型（99%），并且在一名研究参与者中存在一种新的A1C重组菌株。在检查了来自主要临床分离株和数据库衍生序列的所有可用HIV-1C印度序列后，我们发现Tat氨基酸残基的序列保守性很高（92.6 +/- 12%）。此外，特征模式分析确定了Tat中含有印度HIV-1C特有的特征残基的五个氨基酸位置，包括21A、24N、29K、40K和60Q。我们的数据与基于亚单位的Tat HIV-1疫苗开发直接相关。
The trans-activator of transcription (Tat) of HIV-1 plays an important role in viral infection and pathogenesis. We examined the genetic characteristics of exon 1 of the tat gene derived from 102 seropositive subjects from southern India. Database-derived Indian (n = 105) and global (n = 413) HIV-1C sequences were also used for viral epidemiological signature pattern analysis in the Tat open reading frame (ORF). We identified HIV-1C as the most predominant genetic subtype (99%) and the presence of a novel A1C recombinant strain in one study participant. After examining all the available HIV-1C Indian sequences from primary clinical isolates and database-derived sequences, we found a high level of sequence conservation (92.6 +/- 12%) within Tat amino acid residues. Furthermore, signature pattern analysis identified five amino acid positions in Tat that contained signature residues unique for Indian HIV-1C consisting of 21A, 24N, 29K, 40K, and 60Q. Our data have direct relevance for subunit-based Tat HIV-1 vaccine development.