Synthesis of the heteropolysaccharide O antigen of Escherichia coli O52 requires an ABC transporter:: Structural and genetic evidence
Synthesis of the heteropolysaccharide O antigen of Escherichia coli O52 requires an ABC transporter:: Structural and genetic evidence
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DOI：
10.1128/jb.186.14.4510-4519.2004
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发表时间：
2004-07-01
影响因子：
3.2
通讯作者：
Wang, L
中科院分区：
文献类型：
--
作者：
Feng, L;Senchenkova, SN;Wang, L
研究了大肠杆菌 O52 O 抗原的结构和遗传组织。通过糖和甲基化分析以及核磁共振波谱鉴定，大肠杆菌 O52 的 O 抗原具有部分 O-乙酰化的二糖重复单元（O 单元），其中含有 D-呋喃糖和 6-脱氧-D-吡喃甘露糖，以及少量的 6-脱氧-3-O-甲基己糖（最有可能是 3-O-甲基岩藻糖）。大肠杆菌 O52 的 O 抗原基因簇位于 galF 和 gnd 基因之间，被发现包含用于合成 O 抗原成分、糖转移酶基因和 ABC-2 转运蛋白基因的推定基因。进一步的分析证实，O52 采用 ATP 结合盒 (ABC) 转运蛋白依赖性途径进行 O 单元的易位和聚合。这是首次报道 ABC 转运蛋白参与大肠杆菌中杂多糖 O 抗原的易位。还鉴定了大肠杆菌 O52 特异的基因。
The structural and genetic organization of the Escherichia coli O52 O antigen was studied. As identified by sugar and methylation analysis and nuclear magnetic resonance spectroscopy, the O antigen of E. coli O52 has a partially O-acetylated disaccharide repeating unit (O unit) containing D-fucofuranose and 6-deoxy-D-mannoheptopyranose, as well as a minor 6-deoxy-3-O-methylhexose (most likely, 3-O-methylfucose). The O-antigen gene cluster of E. coli O52, which is located between the galF and gnd genes, was found to contain putative genes for the synthesis of the O-antigen constituents, sugar transferase genes, and ABC-2 transporter genes. Further analysis confirmed that O52 employs an ATP-binding cassette (ABC) transporter-dependent pathway for translocation and polymerization of the O unit. This is the first report of an ABC transporter being involved in translocation of a heteropolysaccharide O antigen in E. coli. Genes specific for E. coli O52 were also identified.